健康研究
健康研究
건강연구
HEALTH RESEARCH
2014年
1期
22-25
,共4页
王文天%邢旺兴%金乐红%唐婷
王文天%邢旺興%金樂紅%唐婷
왕문천%형왕흥%금악홍%당정
头孢噻肟%舒巴坦%系数倍率法%含量测定
頭孢噻肟%舒巴坦%繫數倍率法%含量測定
두포새우%서파탄%계수배솔법%함량측정
cefotaxime%sulbactam%K-ratio method%content determination
目的:建立系数倍率法测定注射用头孢噻肟钠舒巴坦钠的含量。方法头孢噻肟和舒巴坦标准溶液在200~400 nm紫外区进行波长扫描,确定头孢噻肟和舒巴坦的最大吸收波长235 nm和258 nm为测定波长,并选择适当的测定波长对和确定K值。以0.1 mol/L NaOH为空白溶剂,利用系数倍率法测定头孢噻肟钠和舒巴坦钠的含量。结果头孢噻肟在4~16μg/mL范围内线性关系良好,回归方程为ΔA=0.01956C+0.00461(r=0.9998),精密度试验RSD=0.52%,平均回收率为101.5%,RSD=1.4%;舒巴坦在2~8μg/mL范围内线性关系良好,回归方程为ΔA=0.06566C+0.00425(r=0.9998),精密度试验RSD=0.33%,平均回收率为103.0%,RSD=1.4%;稳定性试验显示结果在2.5小时内稳定。结论系数倍率法简便、准确、重复性好,可用于注射用头孢噻肟钠舒巴坦钠含量的快速测定。
目的:建立繫數倍率法測定註射用頭孢噻肟鈉舒巴坦鈉的含量。方法頭孢噻肟和舒巴坦標準溶液在200~400 nm紫外區進行波長掃描,確定頭孢噻肟和舒巴坦的最大吸收波長235 nm和258 nm為測定波長,併選擇適噹的測定波長對和確定K值。以0.1 mol/L NaOH為空白溶劑,利用繫數倍率法測定頭孢噻肟鈉和舒巴坦鈉的含量。結果頭孢噻肟在4~16μg/mL範圍內線性關繫良好,迴歸方程為ΔA=0.01956C+0.00461(r=0.9998),精密度試驗RSD=0.52%,平均迴收率為101.5%,RSD=1.4%;舒巴坦在2~8μg/mL範圍內線性關繫良好,迴歸方程為ΔA=0.06566C+0.00425(r=0.9998),精密度試驗RSD=0.33%,平均迴收率為103.0%,RSD=1.4%;穩定性試驗顯示結果在2.5小時內穩定。結論繫數倍率法簡便、準確、重複性好,可用于註射用頭孢噻肟鈉舒巴坦鈉含量的快速測定。
목적:건립계수배솔법측정주사용두포새우납서파탄납적함량。방법두포새우화서파탄표준용액재200~400 nm자외구진행파장소묘,학정두포새우화서파탄적최대흡수파장235 nm화258 nm위측정파장,병선택괄당적측정파장대화학정K치。이0.1 mol/L NaOH위공백용제,이용계수배솔법측정두포새우납화서파탄납적함량。결과두포새우재4~16μg/mL범위내선성관계량호,회귀방정위ΔA=0.01956C+0.00461(r=0.9998),정밀도시험RSD=0.52%,평균회수솔위101.5%,RSD=1.4%;서파탄재2~8μg/mL범위내선성관계량호,회귀방정위ΔA=0.06566C+0.00425(r=0.9998),정밀도시험RSD=0.33%,평균회수솔위103.0%,RSD=1.4%;은정성시험현시결과재2.5소시내은정。결론계수배솔법간편、준학、중복성호,가용우주사용두포새우납서파탄납함량적쾌속측정。
Objective To formulate a method for determining the contents Cefotaxime Sodium and Sulbactam Sodium for injection using K-ratio Method.Method Use ultraviolet wavelength to scan the standard solution of cefotaxime and sulbactam to get the scanning spectrum .Then determined the maximum absorption wavelength of cefotaxime and sulbactam as measuring wavelength which were 235 nm and 258 nm, and determined the wavelength pair and K , then established a standard curve to determine the content of cefotaxime sodium and sulbactam sodium using K -ratio method .Findings The experimental process was steady in 2.5 hours.The linear range of cefotaxime was 4 ~16μg/mL, ΔA=0.01956C+0. 00461(r=0.9998.The precision of cefotaxime was good (RSD=0.52%,n=6); The average recovery was 101.5% (RSD =1.4%,n =9) .The linear range of sulbactam was 2 ~8μg/mL, ΔA =0.06566C +0.00425(r =0.9998.Theprecision of sulbactam was good (RSD =0.33%,n =6); The average recovery was 103.0% (RSD =1.4%,n =9). Conclusion K-ratio Method is a simple,accurate, reproducible and it can be used to determinate the contents ofcefotaxime sodium and sulbactam sodium quickly .