实验与检验医学
實驗與檢驗醫學
실험여검험의학
EXPERIMENTAL AND LABORATORY MEDICINE
2014年
1期
1-4
,共4页
杭亚平(综述)%胡龙华%王小中(审校)
杭亞平(綜述)%鬍龍華%王小中(審校)
항아평(종술)%호룡화%왕소중(심교)
MALDI-TOF MS%微生物鉴定%感染性疾病%应用进展
MALDI-TOF MS%微生物鑒定%感染性疾病%應用進展
MALDI-TOF MS%미생물감정%감염성질병%응용진전
Matrix assisted laser desorption/ionization time of flight mass spectrometry%Microbial identification%Infectious disease%Application progress
质谱(mass spectrometry,MS)技术，尤其是基质辅助激光解吸电离飞行时间质谱(matrix assisted laser desorption/ion-ization time of flight mass spectrometry，MALDI-TOF MS)，具有简便、快速及特异地分析微生物生物大分子标记物质谱图，从而实现临床微生物检验中细菌、真菌及病毒在属、种、株水平上的鉴定。MS在菌血症、尿路感染、毒素检测及耐药监测方面显示出巨大的优势，有望成为临床微生物实验室感染性疾病常用诊断方法。
質譜(mass spectrometry,MS)技術，尤其是基質輔助激光解吸電離飛行時間質譜(matrix assisted laser desorption/ion-ization time of flight mass spectrometry，MALDI-TOF MS)，具有簡便、快速及特異地分析微生物生物大分子標記物質譜圖，從而實現臨床微生物檢驗中細菌、真菌及病毒在屬、種、株水平上的鑒定。MS在菌血癥、尿路感染、毒素檢測及耐藥鑑測方麵顯示齣巨大的優勢，有望成為臨床微生物實驗室感染性疾病常用診斷方法。
질보(mass spectrometry,MS)기술，우기시기질보조격광해흡전리비행시간질보(matrix assisted laser desorption/ion-ization time of flight mass spectrometry，MALDI-TOF MS)，구유간편、쾌속급특이지분석미생물생물대분자표기물질보도，종이실현림상미생물검험중세균、진균급병독재속、충、주수평상적감정。MS재균혈증、뇨로감염、독소검측급내약감측방면현시출거대적우세，유망성위림상미생물실험실감염성질병상용진단방법。
Mass-spectrometric techniques, especially the matrix assisted laser desorption/ionization time of flight mass spec-trometry (MALDI-TOF MS) analyze the mass spectrum of the biomarkers of microorganism conveniently, rapidly and distinctly , demonstrating the identification of bacteria and fungus at level of genus,species and strain. In bacteremia, urinary tract infections, toxin detection and drug resistance monitoring,MS shows great advantage, making it commonly-used diagnostic method of infec-tious diseases for clinical microbiology laboratory hopefully.
