CAJ | 학술논문

研究了南美白对虾苗中4种硝基呋喃代谢物呋喃唑酮代谢物（AOZ）、呋喃它酮代谢物（AMOZ）、呋喃妥因代谢物（AHD）、呋喃西林代谢物（SEM）的高效液相色谱-串联质谱（HPLC-MS/MS）测定方法。以水解衍生并添加4种同位素内标的方法对样品进行处理，补偿了衍生化效率，提高了定量的准确性。实验结果表明，AOZ， AMOZ的检出限为0.10μg/kg，SEM，AHD的检出限为0.25μg/kg；AOZ，AMOZ的定量下限为0.40μg/kg， SEM，AHD的定量下限为0.50μg/kg。方法加标回收率在88.2％～97.6％之间，相对标准偏差在4.6％～8.1％（n=6)之间。该法检测灵敏度高，测量结果的精密度和准确度满足药物残留检测要求。
연구료남미백대하묘중4충초기부남대사물부남서동대사물（AOZ）、부남타동대사물（AMOZ）、부남타인대사물（AHD）、부남서림대사물（SEM）적고효액상색보-천련질보（HPLC-MS/MS）측정방법。이수해연생병첨가4충동위소내표적방법대양품진행처리，보상료연생화효솔，제고료정량적준학성。실험결과표명，AOZ， AMOZ적검출한위0.10μg/kg，SEM，AHD적검출한위0.25μg/kg；AOZ，AMOZ적정량하한위0.40μg/kg， SEM，AHD적정량하한위0.50μg/kg。방법가표회수솔재88.2％～97.6％지간，상대표준편차재4.6％～8.1％（n=6)지간。해법검측령민도고，측량결과적정밀도화준학도만족약물잔류검측요구。
High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneously analyzing the metabolites of four nitrofuran antibiotics such as furazolidone,furaltadone, nitrofurazone and nitrofurantoin in Penaeus vannamei seed. Four isotope internal standards were utilized to improve the quantitative precision. The detection limits (LODs) were 0.10μg/kg for the metabolite of furazolidone and furaltadone, and 0.25μg/kg for the metabolite of nitrofurazone and nitrofurantoin. The quantitative limits were 0.40μg/kg for the metabolite of furazolidone and furaltadone, and 0.50μg/kg for the metabolite of nitrofurazone and nitrofurantoin. The recoveries calibrated by internal standard ranged from 88.2%to 97.6%with the relative standard deviations of 4.6%-8.1%(n=6). The method has high detection sensitivity and the detection result can meet the requirements of drug residue detection.