CAJ | 학술논문

建立超声辅助萃取-固相净化-高效液相色谱法测定乳饮料中硫氰酸钠的方法，重点研究了乳饮料的样品前处理方法，采用乙腈加三氯乙酸作为蛋白沉淀剂，有效实现对各类乳饮料的样品中蛋白质的沉淀和硫氰酸钠的提取。方法在0.05～50 mg·kg-1具有良好的线性关系，硫氰酸钠的日内精密度3.29%～5.56%，日间精密度4.32%～6.45%；不同乳饮料的回收率范围变化为87.73%～106.37%。此方法中硫氰酸钠检出限是0.024 mg·kg-1。
건립초성보조췌취-고상정화-고효액상색보법측정유음료중류청산납적방법，중점연구료유음료적양품전처리방법，채용을정가삼록을산작위단백침정제，유효실현대각류유음료적양품중단백질적침정화류청산납적제취。방법재0.05～50 mg·kg-1구유량호적선성관계，류청산납적일내정밀도3.29%～5.56%，일간정밀도4.32%～6.45%；불동유음료적회수솔범위변화위87.73%～106.37%。차방법중류청산납검출한시0.024 mg·kg-1。
Ultrasonic-assisted solid phase extraction method was developed to determine sodium thiocyanate of dairy beverage by high-performance liquid chromatography,which studied importantly the sample pretreatment. Using acetonitrile and trichloroacetic acid as precipitate protein, the precipitate protein and extraction of the sodium thiocyanate contaminant could be achieved effectively,and the calibration curve was linear in the range of 0.05-50 mg·kg-1,intra-day precision of 3.29%to 5.56%,inter-day precision of sodium thiocyanate of 4.32% to 6.45% and the recoveries of different dairy beverage range of 87.73% to 106.37%. Sodium thiocyanate of detection limits was 0.024 mg·kg-1 by this method .