黑龙江八一农垦大学学报
黑龍江八一農墾大學學報
흑룡강팔일농은대학학보
JOURNAL OF HEILONGJIANG AUGUST FIRST LAND RECLAMATION UNIVERSITY
2014年
1期
36-39
,共4页
高俊峰%王卓林%侯美如%李桂伟%王丽坤
高俊峰%王卓林%侯美如%李桂偉%王麗坤
고준봉%왕탁림%후미여%리계위%왕려곤
中药%兔球虫%卵囊%孢子化率
中藥%兔毬蟲%卵囊%孢子化率
중약%토구충%란낭%포자화솔
Chinese herbal medicine%rabbit coccidia%oocysts%sporulated rate
研究单味中药在不同时间及不同浓度下对兔球虫卵囊孢子化抑制效果。首先,在同浓度单味中药培养的条件下进行观察,发现随着时间的延长,除鸦胆子中卵囊孢子化率没有升高外,其余中药球虫卵囊孢子化率均有不同程度的升高;鸦胆子对兔球虫卵囊孢子化有显著的抑制作用,仙鹤草、常山、青蒿、马鞭草、使君子和柴胡有一定的抑制作用;各组洗去中药继续培养3 d,发现各组卵囊孢子化率均呈上升趋势。这表明,虽然有些中药对兔球虫孢子化有一定的抑制作用,却不能杀灭卵囊。不同浓度中药抑制实验结果表明,仙鹤草、常山、青蒿、鸦胆子随浓度增高孢子化率改变明显,100%浓度抑制球虫卵囊孢子化的效果优于50%和25%浓度,而马鞭草、使君子、柴胡随浓度变化改变不明显。
研究單味中藥在不同時間及不同濃度下對兔毬蟲卵囊孢子化抑製效果。首先,在同濃度單味中藥培養的條件下進行觀察,髮現隨著時間的延長,除鴉膽子中卵囊孢子化率沒有升高外,其餘中藥毬蟲卵囊孢子化率均有不同程度的升高;鴉膽子對兔毬蟲卵囊孢子化有顯著的抑製作用,仙鶴草、常山、青蒿、馬鞭草、使君子和柴鬍有一定的抑製作用;各組洗去中藥繼續培養3 d,髮現各組卵囊孢子化率均呈上升趨勢。這錶明,雖然有些中藥對兔毬蟲孢子化有一定的抑製作用,卻不能殺滅卵囊。不同濃度中藥抑製實驗結果錶明,仙鶴草、常山、青蒿、鴉膽子隨濃度增高孢子化率改變明顯,100%濃度抑製毬蟲卵囊孢子化的效果優于50%和25%濃度,而馬鞭草、使君子、柴鬍隨濃度變化改變不明顯。
연구단미중약재불동시간급불동농도하대토구충란낭포자화억제효과。수선,재동농도단미중약배양적조건하진행관찰,발현수착시간적연장,제아담자중란낭포자화솔몰유승고외,기여중약구충란낭포자화솔균유불동정도적승고;아담자대토구충란낭포자화유현저적억제작용,선학초、상산、청호、마편초、사군자화시호유일정적억제작용;각조세거중약계속배양3 d,발현각조란낭포자화솔균정상승추세。저표명,수연유사중약대토구충포자화유일정적억제작용,각불능살멸란낭。불동농도중약억제실험결과표명,선학초、상산、청호、아담자수농도증고포자화솔개변명현,100%농도억제구충란낭포자화적효과우우50%화25%농도,이마편초、사군자、시호수농도변화개변불명현。
To investigate the inhibition effect of the different concentration of single Chinese herbal medicine on the sporulating of rabbit coccidia oocysts at different times,a series of experiments were performed. Firstly,Under the same concentration culture conditions,the sporulated rate of rabbit coccidia oocysts gradually went up with time in all choosed Chinese herbal medicin,except for fructus bruceae. In other words,suppression effect of fructus bruceae was significant,and herba agrimoniae,radix dichroae,herba artemisiae annuae,herba verbenae,fructus quisqualis,radix bupleuri had a certain suppression effect. Then Chinese herbal medicine was washed and cultured for 3 days,and the sporulated rate of rabbit coccidia oocysts had been on the rise in all groups,which showed that some Chinese herbs had some certain suppression effect on the sporulating of rabbit coccidia oocysts,but they did not effectively eliminate the oocysts. Additionally,inhibition test was performed under different concentration culture conditions.The results showed that the sporulated rate of oocysts were changed significantly with increase in the concentration by herba agrimoniae, radix dichroae,herba artemisiae annuae and fructus bruceae,especially the suppression effect of 100% concentration was better than 50% and 25%. By contrast,the sporulated rate of oocysts increased were not significantly with the above concentration by herba verbenae,fructus quisqualis and radix bupleuri.