广西植物
廣西植物
엄서식물
GUIHAIA
2014年
1期
130-134
,共5页
刘世彪%谭秀梅%彭小列%杨鹏%吕江明
劉世彪%譚秀梅%彭小列%楊鵬%呂江明
류세표%담수매%팽소렬%양붕%려강명
绞股蓝%种子油%理化性质%脂肪酸组成%急性毒性实验
絞股藍%種子油%理化性質%脂肪痠組成%急性毒性實驗
교고람%충자유%이화성질%지방산조성%급성독성실험
Gynostemma pentaphyllum%seed oil%physicochemical property%fatty acid composition%acute toxicity
以石油醚为提取剂、料液比为1∶7,进行单因子和正交试验及急性毒性实验.结果表明：超声波辅助提取绞股蓝种子油的最适工艺条件为提取的温度60℃、功率200 W和时间50 min,此条件下种子油提取率为28．13％；绞股蓝种子油的密度为0．895 g??mL-1、折光值1．518 nD20、酸值0．85和皂化值180．2 mg KOH??g-1、碘值135．7 g I2??100g-1,为优良的干性油；绞股蓝种子油含有10种脂肪酸成分,主要为亚麻酸(80．49％)、油酸(8．05％)、亚油酸(7．08％)、硬脂酸(1．79％)和棕榈酸(1．28％),不饱和脂肪酸质量分数达总脂肪酸的96．48％；以剂量65 mL??(kg??d)-1的绞股蓝种子油灌胃小鼠,在连续14 d的实验期内,没有发现小鼠的中毒症状和死亡现象.初步判定绞股蓝种子油无急性毒性,有望作为营养保健油源进一步开发.
以石油醚為提取劑、料液比為1∶7,進行單因子和正交試驗及急性毒性實驗.結果錶明：超聲波輔助提取絞股藍種子油的最適工藝條件為提取的溫度60℃、功率200 W和時間50 min,此條件下種子油提取率為28．13％；絞股藍種子油的密度為0．895 g??mL-1、摺光值1．518 nD20、痠值0．85和皂化值180．2 mg KOH??g-1、碘值135．7 g I2??100g-1,為優良的榦性油；絞股藍種子油含有10種脂肪痠成分,主要為亞痳痠(80．49％)、油痠(8．05％)、亞油痠(7．08％)、硬脂痠(1．79％)和棕櫚痠(1．28％),不飽和脂肪痠質量分數達總脂肪痠的96．48％；以劑量65 mL??(kg??d)-1的絞股藍種子油灌胃小鼠,在連續14 d的實驗期內,沒有髮現小鼠的中毒癥狀和死亡現象.初步判定絞股藍種子油無急性毒性,有望作為營養保健油源進一步開髮.
이석유미위제취제、료액비위1∶7,진행단인자화정교시험급급성독성실험.결과표명：초성파보조제취교고람충자유적최괄공예조건위제취적온도60℃、공솔200 W화시간50 min,차조건하충자유제취솔위28．13％；교고람충자유적밀도위0．895 g??mL-1、절광치1．518 nD20、산치0．85화조화치180．2 mg KOH??g-1、전치135．7 g I2??100g-1,위우량적간성유；교고람충자유함유10충지방산성분,주요위아마산(80．49％)、유산(8．05％)、아유산(7．08％)、경지산(1．79％)화종려산(1．28％),불포화지방산질량분수체총지방산적96．48％；이제량65 mL??(kg??d)-1적교고람충자유관위소서,재련속14 d적실험기내,몰유발현소서적중독증상화사망현상.초보판정교고람충자유무급성독성,유망작위영양보건유원진일보개발.
Based on petroleum ether as extraction solvent and ratio of material to solution 1∶7,the ultrasonic-assisted extraction process on seed oil of Gynostemma pentaphyllum was optimized by single factor test and orthogonal test. The optimal ultrasonic-assisted conditions were as follows:extraction temperature 60 ℃,ultrasonic power 200 W and extraction time 50 min,under which the extraction rate of seed oil could reach 28.13%.Physicochemical properties of the seed oil including density,dioptre,acid value,saponification value and iodine value were respectively 0.895 g??mL-1,1.518 nD20,0.85,180.2 mg KOH??g-1 and 135.7 g I2??100g-1,which revealed thatG.pentaphyllum seed oil was a fine drying oil.GC-MS analysis revealed that there were 10 kinds of fatty acids in G.pentaphyllum seed oil,and the mass fraction were linolenic acid(80.49%),oleic acid(8.05%),linoleic acid(7.08%),stearic acid (1.79%)and palmitic acid(1.28%).The unsaturated fatty acids accounted for 96.48% of total fatty acids.In the acute toxicity test,a dose of 65 mL??(kg?? d)-1 of seed oil were filled into mice’s stomach,and no evident poisoning symptoms and animal death were detected after 14 d continuous observation,which preliminarily indicated that G. pentaphyllum seed oil was of nontoxicity,and expected to be developed as a resource of health oil.
