CAJ | 학술논문

以莴苣幼苗为材料,检测了分离自细果角茴香的生物碱对莴苣幼苗根生长及根毛发育的影响。结果表明：50~200μmol??L-1浓度范围内,8-甲氧基二氢血根碱对莴苣幼苗根长显著抑制；10、20和30μmol??L-18-甲氧基二氢血根碱对莴苣幼苗根毛的长度和数量有显著的抑制作用,且抑制作用均表现了浓度依赖性。通过对莴苣根尖细胞有丝分裂研究发现,50μmol??L-1的8-甲氧基二氢血根碱能显著抑制根尖细胞的有丝分裂,而且莴苣幼苗净增值率与根尖细胞的有丝分裂之间呈正相关,证明8-甲氧基二氢血根碱主要通过抑制根尖细胞有丝分裂对莴苣根的生长产生影响。
이와거유묘위재료,검측료분리자세과각회향적생물감대와거유묘근생장급근모발육적영향。결과표명：50~200μmol??L-1농도범위내,8-갑양기이경혈근감대와거유묘근장현저억제；10、20화30μmol??L-18-갑양기이경혈근감대와거유묘근모적장도화수량유현저적억제작용,차억제작용균표현료농도의뢰성。통과대와거근첨세포유사분렬연구발현,50μmol??L-1적8-갑양기이경혈근감능현저억제근첨세포적유사분렬,이차와거유묘정증치솔여근첨세포적유사분렬지간정정상관,증명8-갑양기이경혈근감주요통과억제근첨세포유사분렬대와거근적생장산생영향。
Effects of 8-methoxy dihydrosanguinarine isolated from Hypecoum leptocapum on the root growth and root hair development of Lactuca sativa seedlings were studied.The results showed that the root growth was signifi-cantly inhibited by 8-methoxy dihydrosanguinarine at the concentration of 50-200μmol??L-1;the inhibitory effect on root hairs increased with concentration ranging from 10 to 30μmol??L-1 .The inhibitory effects of tested compound on the length and number of root hairs showed a dose-dependent manner.Moreover,the mitosis of apical cells could be inhibited by 8-methoxy dihydrosanguinarine at the concentrations above 50μmol??L-1 and revealed a positive linear regression with root NGR.Thus,the effect of 8-methoxy dihydrosanguinarine on Lactuca sativa seedlings mainly ex-plored on the inhibition of the mitotic activity.