CAJ | 학술논문

水鳖科(Hydrocharitaceae)海菜花(Ottelia acuminata)是中国西南地区特有的水生单子叶植物.基于AFLP技术的磁珠富集快速分离技术(Fast Isolation by AFLP of Sequences Containing Repeats,FIAS-CO),共筛选出9对多态性引物并对3个居群45个个体进行分析.结果表明：三个居群的等位基因数目为1~3个,观测杂合度从0．000~0．933,期望杂合度从0．000~0．605.这些筛选出的微卫星引物将用于海菜花后续的谱系地理学和生态遗传学研究.
수별과(Hydrocharitaceae)해채화(Ottelia acuminata)시중국서남지구특유적수생단자협식물.기우AFLP기술적자주부집쾌속분리기술(Fast Isolation by AFLP of Sequences Containing Repeats,FIAS-CO),공사선출9대다태성인물병대3개거군45개개체진행분석.결과표명：삼개거군적등위기인수목위1~3개,관측잡합도종0．000~0．933,기망잡합도종0．000~0．605.저사사선출적미위성인물장용우해채화후속적보계지이학화생태유전학연구.
Ottelia acuminata (Gagnepain)Dandy (Hydrocharitaceae),is a submerged monocot endemic to southwest-ern China.Using the fast isolation by AFLP of sequences containing repeats (FIASCO)protocol,nine polymorphic microsatellite loci were identified by the genotyping of forty-five individuals from three natural populations.The num-ber of alleles (NA)per locus within populations varied from one to three.The observed and expected heterozygosities ranged from 0.000 to 0.933 and 0.000 to 0.605,respectively.These microsatellite primers can be used in future studies on the phylogeography and ecological genetics of O.acuminata.