CAJ | 학술논문

利用JSM-6360LV型扫描电镜和JEM-1010型透射电镜，观察了南美蟛蜞菊、蟛蜞菊及其自然杂交种新近成熟和老熟叶片的解剖结构及叶绿体超微结构。结果表明：遮荫后该杂交种与其亲本新近成熟叶片均表现为上下表皮气孔密度、叶片总厚度及上下表皮厚度、栅栏组织、海绵组织厚度减小，叶绿体肿胀变形，基粒片层垛叠程度增加，淀粉粒增多变大；遮荫后杂交种老熟叶片总厚度及上表皮、栅栏组织、海绵组织厚度增加，入侵种的下表皮厚度及本地种的上表皮厚度增加，叶绿体超微结构在遮荫后均出现严重损伤，基粒片层类囊体结构边缘溶解等。说明三种蟛蜞菊属物种及各物种不同叶龄叶片对弱光条件的响应存在差异；杂交种叶片显微及超微结构在不同光照下的变化介于亲本之间，对遮荫环境能较好适应。
이용JSM-6360LV형소묘전경화JEM-1010형투사전경，관찰료남미팽기국、팽기국급기자연잡교충신근성숙화노숙협편적해부결구급협록체초미결구。결과표명：차음후해잡교충여기친본신근성숙협편균표현위상하표피기공밀도、협편총후도급상하표피후도、책란조직、해면조직후도감소，협록체종창변형，기립편층타첩정도증가，정분립증다변대；차음후잡교충노숙협편총후도급상표피、책란조직、해면조직후도증가，입침충적하표피후도급본지충적상표피후도증가，협록체초미결구재차음후균출현엄중손상，기립편층류낭체결구변연용해등。설명삼충팽기국속물충급각물충불동협령협편대약광조건적향응존재차이；잡교충협편현미급초미결구재불동광조하적변화개우친본지간，대차음배경능교호괄응。
The microstructure and the chloroplast ultrastructure of the primary fully expanded mature and senescent leaves of Wedelia trilobata,W.chinensis and their hybrid under shade and full light have been investigated using scanning electron microscope(SEM,JSM-6360LV)and transmission electron microscope(TEM,JEM-1010).In con-trast to full light treatment,the primary fully expanded mature leaves of the three species under shade treatment had reduced stomatal density of both upper and lower epidermises,and smaller leaf thicknesses and thicknesses of upper and lower epidermises,palisade tissue and spongy tissue.Moreover,the chloroplast of the primary fully expanded ma-ture leaves,the stacking of grana lamellae increased and the number of the starch grain increased.However,the effects of shade on the microstructure of the senescent leaves were different to that found in primary leaves.For hy-brids,the thicknesses of the upper epidermis,the palisade tissue,the spongy tissue and the whole leaves increased.Al-so,the thickness of the lower epidermis of the invasive species(W.trilobata)and the thickness of the upper epidermis of the native species(W.chinensis)increased.Moreover,the ultrastructure of their chloroplasts had more severe im-pairment under shade treatment,e.g.the grana lamellae had edge-lysis.Conclusively,the response in leaf microstruc-ture and chloroplast ultrastructure varied among different Wedelia species and different leaf developmental stages within the same species;the hybrid showed intermediate response between its parent species.