广西植物
廣西植物
엄서식물
GUIHAIA
2014年
1期
15-18,129
,共5页
宋春凤%刘启新%周义峰%吴宝成%董晓宇
宋春鳳%劉啟新%週義峰%吳寶成%董曉宇
송춘봉%류계신%주의봉%오보성%동효우
伞形科%珊瑚菜%SRAP%居群
傘形科%珊瑚菜%SRAP%居群
산형과%산호채%SRAP%거군
Apiaceae%Glehnia littoralis%SRAP%population
利用SRAP对伞形科单种属珊瑚菜7个野生居群和1个栽培居群进行了研究.结果表明:共筛选出8个引物组合,在珊瑚菜8个居群中共扩增出168条条带,其中多态性条带为118条,多态性比率为70.23%;平均每对引物扩增的多态性条带为14.75.各居群之间珊瑚菜遗传相似性系数范围为0.8306~0.9836,遗传距离范围为0.0165~0.1856.聚类分析表明,以相似性系数大于0.8并结合地理分布来看,所研究的野生珊瑚菜居群可以大体分为3类,辽宁大连的野生居群为一类,山东威海-山东青岛的居群为一类,而山东日照-广州深圳之间的为一类.
利用SRAP對傘形科單種屬珊瑚菜7箇野生居群和1箇栽培居群進行瞭研究.結果錶明:共篩選齣8箇引物組閤,在珊瑚菜8箇居群中共擴增齣168條條帶,其中多態性條帶為118條,多態性比率為70.23%;平均每對引物擴增的多態性條帶為14.75.各居群之間珊瑚菜遺傳相似性繫數範圍為0.8306~0.9836,遺傳距離範圍為0.0165~0.1856.聚類分析錶明,以相似性繫數大于0.8併結閤地理分佈來看,所研究的野生珊瑚菜居群可以大體分為3類,遼寧大連的野生居群為一類,山東威海-山東青島的居群為一類,而山東日照-廣州深圳之間的為一類.
이용SRAP대산형과단충속산호채7개야생거군화1개재배거군진행료연구.결과표명:공사선출8개인물조합,재산호채8개거군중공확증출168조조대,기중다태성조대위118조,다태성비솔위70.23%;평균매대인물확증적다태성조대위14.75.각거군지간산호채유전상사성계수범위위0.8306~0.9836,유전거리범위위0.0165~0.1856.취류분석표명,이상사성계수대우0.8병결합지리분포래간,소연구적야생산호채거군가이대체분위3류,료녕대련적야생거군위일류,산동위해-산동청도적거군위일류,이산동일조-엄주심수지간적위일류.
Sequence-related amplified polymorphism (SRAP)was used to analyze genetic relationships of eight popu-lations of Glehnia littoralis F.Schmidt ex Miquel in China.Among them,seven populations were wild plants and one was cultivar plant.With eight SRAP primer combinations,1 6 8 loci were identified.Among them,1 1 8 were polymor-phic and accounted for 70.23% of total amplified loci,showing a high polymorphism.The genetic similarity among these eight populations ranged from 0.830 6 to 0.983 6 and the genetic diversity was 0.0165-0.1856.Based on the results of cluster and principal coordinate analysis,the eight populations of G.littoralis were divided into three groups.The population from Dalian of Liaoling Province was confirmed as Group A,while populations from Shan-dong Province (excluding Rizhao)were clustered into Group B.Group C comprised populations from Rizhao of Shan-dong Province to Shenzhen,Guangzhou.