广西植物
廣西植物
엄서식물
GUIHAIA
2014年
1期
10-14
,共5页
细叶云南松%SSR分子标记%遗传多样性
細葉雲南鬆%SSR分子標記%遺傳多樣性
세협운남송%SSR분자표기%유전다양성
Pinus yunnanensis var.tenuifolia%SSR molecular marker%genetic diversity
利用12对SSR引物对三个不同种源的细叶云南松群体遗传多样性进行研究.结果表明：共检测到13个位点37个等位基因,每个位点平均观察等位基因数(A)为2．85,多态率为100％；平均有效等位基因数(Ne)1．45.各群体内的有效等位基因数平均为1．447,观察杂合度平均为0．341,期望杂合度平均为0．281.三个群体的Nei’s 基因多样度指标的变化范围为0．256~0．297,Shannon多样性指数变化范围为0．448~0．484,各群体间的多态性水平差异不大.细叶云南松群体间的基因分化系数(Gst)为0．089,群体间的遗传分化水平较低,大部分变异均存在群体内.细叶云南松群体间的基因流(Nm)在不同位点的变化范围从4．693~122．189,平均为11．17.说明细叶云南松群体间存在比较充分的基因交流.
利用12對SSR引物對三箇不同種源的細葉雲南鬆群體遺傳多樣性進行研究.結果錶明：共檢測到13箇位點37箇等位基因,每箇位點平均觀察等位基因數(A)為2．85,多態率為100％；平均有效等位基因數(Ne)1．45.各群體內的有效等位基因數平均為1．447,觀察雜閤度平均為0．341,期望雜閤度平均為0．281.三箇群體的Nei’s 基因多樣度指標的變化範圍為0．256~0．297,Shannon多樣性指數變化範圍為0．448~0．484,各群體間的多態性水平差異不大.細葉雲南鬆群體間的基因分化繫數(Gst)為0．089,群體間的遺傳分化水平較低,大部分變異均存在群體內.細葉雲南鬆群體間的基因流(Nm)在不同位點的變化範圍從4．693~122．189,平均為11．17.說明細葉雲南鬆群體間存在比較充分的基因交流.
이용12대SSR인물대삼개불동충원적세협운남송군체유전다양성진행연구.결과표명：공검측도13개위점37개등위기인,매개위점평균관찰등위기인수(A)위2．85,다태솔위100％；평균유효등위기인수(Ne)1．45.각군체내적유효등위기인수평균위1．447,관찰잡합도평균위0．341,기망잡합도평균위0．281.삼개군체적Nei’s 기인다양도지표적변화범위위0．256~0．297,Shannon다양성지수변화범위위0．448~0．484,각군체간적다태성수평차이불대.세협운남송군체간적기인분화계수(Gst)위0．089,군체간적유전분화수평교저,대부분변이균존재군체내.세협운남송군체간적기인류(Nm)재불동위점적변화범위종4．693~122．189,평균위11．17.설명세협운남송군체간존재비교충분적기인교류.
SSR markers were used to study genetic diversity of tree different nature populations of Pinus yunnanensis var.tenuifolia.The results showed that 13 sites and 37 alleles were detected.For every site,the average value of ob-servation allele number(A)was 2.85,polymorphism rate was 100% and the average values of effective number of al-leles (Ne)was 1.45.The average value of effective number of alleles was 1.447 within each population.P.yunnanen-sis var.tenuifolia had a low genetic diversity level among the three populations,the average observed heterozygosity (Ho)was 0.341 and the average expected gene heterozygosity(He)was 0.281.Nei’s gene diversities (h)at popula-tion level were 0.256 and 0.297.Shannon’s information indexes (I)at population level were 0.448 and 0.484.In addi-tion,the three populations deviated from Hardy-Weinberge quilibrium.The average coefficient gene differentiation (Gst)was 0.089.Most of variation appeared inner population and there was not gene differentiations among popula-tions.The range of gene flow of different gene polymorphism sites was 4.693-122.189,the average number was 1 1 .1 7 ,which indicated that the habitat of P .yunnanensis var.tenuifolia was limited and resulted in the limited natu-ral variation,but there were abundant gene exchanges between each population.
