CAJ | 학술논문

从64对引物组合中筛选出6对谱带清晰、多态性高的引物组合,对63个橄榄品种扩增,共扩增出417条多态性电泳谱带,多态性比率达100％,揭示了橄榄品种的遗传多样性.利用UPGMA对供试橄榄品种聚类,建立AFLP聚类图.结果表明：63份材料之间的遗传相似性系数为0．0606~0．7619,相似系数为0．312时,可将供试材料分为7个品种群,其中第1群包括45个品种,以‘潮阳甜榄’为代表,还可细分成3个组；第2群包括5个品种,以‘大纳甜’为代表；第3群包括4个品种,以‘潮阳三棱’为代表；第4群包括‘土甜’、‘文祠三棱’和‘早花三棱’3个品种；第5群包括‘铁条’和‘细粒’两个品种；第6群包括‘广太甜种’、‘西土’、‘鸡心’和‘车酸1号’4个品种；第7群只有‘凤湖橄榄’1个品种,与其他品种群亲缘关系较远,可能发生变异.
종64대인물조합중사선출6대보대청석、다태성고적인물조합,대63개감람품충확증,공확증출417조다태성전영보대,다태성비솔체100％,게시료감람품충적유전다양성.이용UPGMA대공시감람품충취류,건립AFLP취류도.결과표명：63빈재료지간적유전상사성계수위0．0606~0．7619,상사계수위0．312시,가장공시재료분위7개품충군,기중제1군포괄45개품충,이‘조양첨람’위대표,환가세분성3개조；제2군포괄5개품충,이‘대납첨’위대표；제3군포괄4개품충,이‘조양삼릉’위대표；제4군포괄‘토첨’、‘문사삼릉’화‘조화삼릉’3개품충；제5군포괄‘철조’화‘세립’량개품충；제6군포괄‘엄태첨충’、‘서토’、‘계심’화‘차산1호’4개품충；제7군지유‘봉호감람’1개품충,여기타품충군친연관계교원,가능발생변이.
Screened from 64 pairs of the AFLP primer,six pairs(E/AAC-M/CAT,E/AAC-M/CTT,E/ACA-M/CAA,E/AGG-M/CAA,E/ACC-M/CAA,E/ACT-M/CAT ),which produced clear and reliable polymorphic bands, were selected for analysis genetic relationship and genetic diversity among the 63 Chinese olive cultivars.417 amplifi-cation bands were produced with 100%polymorphic rate.It was indicated that there was rich genetic diversity in Chi-nese olive cultivar germplasm.The genetic relationship of the 6 3 Chinese olive cultivars was analyzed with software NTSYSpc-2.10a,and a UPGMA tree was established.According to this tree,the Chinese olive cultivars in this stud-ied could be divided into 7 cultivar groups with a similar coefficient 0.312,the Group 1 delegated by‘Chaoyang sweet olive’including 45 cultivars,could be divided into 3 types,the Group 2 delegated by‘Danatian’including 5 cultivars, the Group 3 delegated by‘Chaoyang sanleng’including 4 cultivars,the Group 4 including 3 cultivars,the Group 5 in-cluding 2 cultivars,the Group 6 including 4 cultivars,and the Group 7 including one cultivar only,that was ‘Fenghu olive’,which had a long distance in relationship with other cultivars,maybe occurred variation in cultivation practice.