CAJ | 학술논문

植物组织的扫描电镜样品干燥通常采用临界点干燥法和冷冻干燥法，但这些方法存在操作复杂，需要专门仪器等诸多不便。作者在工作中探索了六甲基二硅胺烷（ HMDS）对样品进行干燥，并与冷冻干燥法做了比较。结果表明：在花期柱头和子房等幼嫩组织上，经HMDS干燥后的扫描结果较好且两种方法的结果几乎一致，在幼果期胚珠较为成熟的组织上，HMDS比冷冻干燥法的效果差。
식물조직적소묘전경양품간조통상채용림계점간조법화냉동간조법，단저사방법존재조작복잡，수요전문의기등제다불편。작자재공작중탐색료륙갑기이규알완（ HMDS）대양품진행간조，병여냉동간조법주료비교。결과표명：재화기주두화자방등유눈조직상，경HMDS간조후적소묘결과교호차량충방법적결과궤호일치，재유과기배주교위성숙적조직상，HMDS비냉동간조법적효과차。
Samples of plant tissues for SEM are usually dried by methods of critical point drying and freeze-drying. However, there are limitations with these methods, such as complex operations, specialized equipment requirement and so on. In this paper, the samples were dried with Hexamethyl disilazane (HMDS). The effect was compared with those using the freeze-drying method. The results showed that samples in young tissues, such as the flowering stigma and ovary, could be dried well by both the HMDS method and the freeze-drying method and the effects of them on sample drying are similar. By contrast, the effects of HMDS on drying of mature ovule tissues were worse than that of the freeze-drying method.