CAJ | 학술논문

目的：比较和筛选放线菌扫描电镜样品制备方法。方法：通过培养获得插片、菌饼和发酵液3种待处理样品，根据单因素试验设计原则采用双固定、单固定、磷酸缓冲液清洗、生理盐水清洗、蒸馏水清洗、丙酮脱水、乙醇脱水和直接表面镀金等8种方法对样品进行处理。结果：取样时间直接影响观察样品形态的丰富度；插片样品扫描获得的结果较为理想；不同的固定、清洗和脱水等处理过程也对样品的细节产生了影响。结论：插片样品直接干燥喷金扫描观察是一种简便、快速的方法，对于常规的放线菌观察与鉴定较为实用，也为其它微生物扫描电镜观察提供了参考。
목적：비교화사선방선균소묘전경양품제비방법。방법：통과배양획득삽편、균병화발효액3충대처리양품，근거단인소시험설계원칙채용쌍고정、단고정、린산완충액청세、생리염수청세、증류수청세、병동탈수、을순탈수화직접표면도금등8충방법대양품진행처리。결과：취양시간직접영향관찰양품형태적봉부도；삽편양품소묘획득적결과교위이상；불동적고정、청세화탈수등처리과정야대양품적세절산생료영향。결론：삽편양품직접간조분금소묘관찰시일충간편、쾌속적방법，대우상규적방선균관찰여감정교위실용，야위기타미생물소묘전경관찰제공료삼고。
Objective:To compare and screening actinomycetes scanning electron microscopy sample preparation method. Methods:Eight kinds of processing method( dual fixed, single fixed, washed by phosphate buffer, saline, distilled water, dehydrated by acetone, ethanol, surface plating directly) for processing the samples based on single-factor test designing principles. Results: Sampling time had a direct impact on the morphology richness of the observed sample; insert samples obtained more perfect results; no significant difference between two fixing methods;the samples of different sources should use different cleaning methods;there was no significant difference between the two dehydrating methods. Conclusion:Inserting sample dried directly, sprayed gold and scaned was a reliable, simple, rapid method and was more practical for conventional observetion and identification of actinomycetes and provided a reference for the scanning electron microscope observation of other microorganisms.