CAJ | 학술논문

通过体视显微镜、半薄切片、扫描电子显微镜观察拟南芥DNA从头甲基化酶DRM1，DRM2的双突变体drm1/drm2在愈伤诱导培养基上所诱导的愈伤组织，结果发现：与野生型相比，其种子和根、叶、叶柄等外植体，均具有在同等条件下愈伤组织增多，分裂旺盛的表型。编码拟南芥DNA从头甲基化酶DRM1，DRM2的基因DRM1， DRM2的表达水平也与生长素、细胞分裂素等外源激素浓度相关，由此推测外源激素浓度影响DRM1，DRM2的表达。暗示了DRM1，DRM2可能参与了拟南芥愈伤组织形成过程。
통과체시현미경、반박절편、소묘전자현미경관찰의남개DNA종두갑기화매DRM1，DRM2적쌍돌변체drm1/drm2재유상유도배양기상소유도적유상조직，결과발현：여야생형상비，기충자화근、협、협병등외식체，균구유재동등조건하유상조직증다，분렬왕성적표형。편마의남개DNA종두갑기화매DRM1，DRM2적기인DRM1， DRM2적표체수평야여생장소、세포분렬소등외원격소농도상관，유차추측외원격소농도영향DRM1，DRM2적표체。암시료DRM1，DRM2가능삼여료의남개유상조직형성과정。
By stereo microscope, scanning electron microscope and transmission electron microscope, the microstructure and ultra structure of cells from callus can be observed. Results show that the seedlings of Arabidopsis mutant drm1/drm2, lacking the de novo DNA methyltransferase DRM1, DRM2, can be induced to form more callus compared to the wild type ( WS) on Callus-inducing-Medium (CIM, containing 1 mg/L 2,4-D, 0?1 mg/L KT ). Other different explants of roots, leaves and petioles were used to compare with the seedlings and obtained the same results. The molecular results show the expression levels of genes DRM1,DRM2 coding the de novo DNA methyltransferase DRM1,DRM2 are related to the concentration of hormones (auxin and cytokinin) which can induce the formation of callus. So it can be indicated that DRM1,DRM2 are possibly involved in the callus formation.