CAJ | 학술논문

目的：探讨RANK-RANKL在嘌呤霉素氨基核苷肾病（PAN）大鼠动物模型肾脏中的表达。方法36只SD大鼠被分配为PAN组及对照组，单次静脉注射嘌呤霉素氨基核苷（PA，100 mg/kg）制作PAN动物模型。大鼠于第3、7、14天检测蛋白尿及血肌酐。检测肾脏病理，并分析RANK和RANKL变化。结果（1）在PAN SD大鼠动物模型中，第3、7、14天时蛋白尿与对照组比较有显著差别，第7天达到高峰；（2）用Western blotting及RT-PCR定量检测发现RANK-RANKL蛋白及mRNA在PAN模型组高于对照组；（3）用激光共聚焦技术显示足细胞标记蛋白Synaptopodin与RANK完全重合，提示RANK主要在足细胞表达；（4）免疫电镜发现RANK在PAN模型组明显增多，且主要定位于足突的顶部胞膜和胞质内。结论在PAN SD大鼠动物模型足细胞异常表达RANK-RANKL，提示RANK及RANKL在足细胞损伤中可能发挥重要作用。
목적：탐토RANK-RANKL재표령매소안기핵감신병（PAN）대서동물모형신장중적표체。방법36지SD대서피분배위PAN조급대조조，단차정맥주사표령매소안기핵감（PA，100 mg/kg）제작PAN동물모형。대서우제3、7、14천검측단백뇨급혈기항。검측신장병리，병분석RANK화RANKL변화。결과（1）재PAN SD대서동물모형중，제3、7、14천시단백뇨여대조조비교유현저차별，제7천체도고봉；（2）용Western blotting급RT-PCR정량검측발현RANK-RANKL단백급mRNA재PAN모형조고우대조조；（3）용격광공취초기술현시족세포표기단백Synaptopodin여RANK완전중합，제시RANK주요재족세포표체；（4）면역전경발현RANK재PAN모형조명현증다，차주요정위우족돌적정부포막화포질내。결론재PAN SD대서동물모형족세포이상표체RANK-RANKL，제시RANK급RANKL재족세포손상중가능발휘중요작용。
Objective To investigate RANK-RANKL expression in the kidneys of a rat model of puromycin aminonucleoside nephropathy (PAN). Methods Thirty-six SD rats were randomly divided into PAN model group and normal control group. PAN was induced by a single intravenous injection of 100 mg/kg puromycin aminonucleoside. Serum creatinine and 24-hour urinary protein were measured on days 3, 7, and 14 after the injection, and renal pathologies were assessed with optical and immune transmission electron microscopy. The expression of RANK and RANKL in the kidneys was examined using reverse transcription-ploymerase chain reaction (RT-PCR) and Western blotting. Results The PAN model rats showed massive proteinuria and elevated serum creatinine on day 3, which peaked on day 7. RANK-RANKL protein and mRNA expressions in PAN model group was higher than those in the control group. In the PAN rats, RANK was expressed mainly on the top cell membrane and in the cytoplasm of renal podocytes with a significantly increased expression level compared with that in the control group. Conclusion The PAN rat model shows aberrant RANK and RANKL expressions in the podocytes, indicating their contribution to podocyte injury in PAN.