CAJ | 학술논문

目的：探索miR-124a的靶基因iASPP对神经发育的影响。方法利用在线预测软件TargetScan对miR-124a的靶基因进行预测，结合文献资料提到的与神经早期发育有关的基因进行筛选，我们选择iASPP作为miR-124a的候选靶基因。通过荧光素酶报告基因实验对其进行验证，然后在M17细胞中转染miR-124a过表达质粒，用Western blotting实验检测iASPP蛋白表达水平的变化。利用视黄酸诱导M17细胞作为神经元分化模型，通过过表达或者基因干扰的方法，初步探讨iASPP基因对神经发育的影响。结果miR-124a促进神经突起发育，并且能够与iASPP基因的3'非翻译区（3'UTR）相互作用来抑制其蛋白质的表达；iASPP基因的过表达抑制神经突起的发育并且抑制miR-124a促进神经突起生长的作用。结论miR-124a能够通过抑制iASPP基因的表达来促进神经突起生长。
목적：탐색miR-124a적파기인iASPP대신경발육적영향。방법이용재선예측연건TargetScan대miR-124a적파기인진행예측，결합문헌자료제도적여신경조기발육유관적기인진행사선，아문선택iASPP작위miR-124a적후선파기인。통과형광소매보고기인실험대기진행험증，연후재M17세포중전염miR-124a과표체질립，용Western blotting실험검측iASPP단백표체수평적변화。이용시황산유도M17세포작위신경원분화모형，통과과표체혹자기인간우적방법，초보탐토iASPP기인대신경발육적영향。결과miR-124a촉진신경돌기발육，병차능구여iASPP기인적3'비번역구（3'UTR）상호작용래억제기단백질적표체；iASPP기인적과표체억제신경돌기적발육병차억제miR-124a촉진신경돌기생장적작용。결론miR-124a능구통과억제iASPP기인적표체래촉진신경돌기생장。
Objective To investigate the role of iASPP as the target gene of miR-124a in neural development. Methods Using the online bioinformatical tool (TargetScan) and by reviewing the relevant studies, we selected iASPP as the candidate target gene of miR-124a involved in early-stage neuronal differentiation. Luciferase reporter assay was used to verify the candidate gene. We transfected M17 cells with a miR-124a overexpression plasmid and detected the changes in the protein expression of iASPP using Western blotting. With retinoic acid-induced M17 cells as the neuronal differentiation model, the role of iASPP in early-stage neuronal differentiation was investigated by gene overexpression and gene interference techniques. Results miR-124a inhibited the expression of iASPP in M17 cells by interacting with the 3'UTR of iASPP gene. miR-124a promoted neurite outgrowth of the cells, which was blocked by iASPP overexpression. Conclusion miR-124a promotes neurite outgrowth of M17 cells by inhibiting iASPP expression.