CAJ | 학술논문

研究了泥鳅（ Misgurnus anguillicaudatus）体表黏液对微生物结构蛋白与多糖的降解作用和对微生物生长的影响。采用考马斯亮蓝法和DNS法检测泥鳅黏液与微生物结构大分子混合作用，及黏液上清液中的可溶性蛋白含量和还原糖量。并将泥鳅黏液分别与酵母菌、大肠杆菌、金黄色葡萄球菌、江内船体污损菌FB-a和绿藻混合作用，比较活性黏液和灭活黏液对它们的影响。结果显示，泥鳅黏液对酵母和大肠杆菌的结构蛋白和多糖没有显示出可常规检测到的降解作用；对大肠杆菌和金黄色葡萄球菌以及江内船体污损菌FB-a也没有显示出生长抑制或杀菌作用，两种细菌和FB-a菌株与泥鳅黏液作用后生长正常，甚至生长更旺盛，且菌细胞发生聚集，不易分散；但对遍生绿藻产生明显的生长抑制，与泥鳅粘液作用后，绿藻吸附黏液蛋白成分而发生聚集，贴壁生长明显减弱。
연구료니추（ Misgurnus anguillicaudatus）체표점액대미생물결구단백여다당적강해작용화대미생물생장적영향。채용고마사량람법화DNS법검측니추점액여미생물결구대분자혼합작용，급점액상청액중적가용성단백함량화환원당량。병장니추점액분별여효모균、대장간균、금황색포도구균、강내선체오손균FB-a화록조혼합작용，비교활성점액화멸활점액대타문적영향。결과현시，니추점액대효모화대장간균적결구단백화다당몰유현시출가상규검측도적강해작용；대대장간균화금황색포도구균이급강내선체오손균FB-a야몰유현시출생장억제혹살균작용，량충세균화FB-a균주여니추점액작용후생장정상，심지생장경왕성，차균세포발생취집，불역분산；단대편생록조산생명현적생장억제，여니추점액작용후，록조흡부점액단백성분이발생취집，첩벽생장명현감약。
This paper studied the effects of loach ( Misgurnus anguillicaudatus) body-surface mucus on the degradation of microbial structure protein and polysaccharide and its influence on microbial growth .For the supernate of the mixed loach mucus and microbial′s structure macromolecules , the method of coomassie brilliant blue was used to detect the soluble pro-tein content and the method of DNS was used detect the reducing sugar content .Then mixed loach mucus respectively with E.coli、 staphylococcus aureus, ship fouling bacterium a (FB-a) and algae, compared with the influence of the growth of active mucus and inactivated mucus for two kinds of pathogenic bacteria FB -a and algae.The results showed that the mucus obtained from the loach′s natural life environment showed no conventional detectable degradation on the structural proteins and polysaccharides of yeast and E.coli; loach mucus also showed no growth inhibition or bactericidal effect on E.coli, staphylococcus aureus and ship fouling bacterium a ( FB-a ) , these two kinds of pathogenic bacteria and FB-a strains showed a regular growth after treated with loach mucus , they even grew more vigorously and gathered together so that it′s difficult to disperse; But for living green algae , the mucus showed obvious growth inhibition .Green algae adhered to mu-cous protein and gathered together , then adherent growth decreased significantly .