淡水渔业
淡水漁業
담수어업
FRESHWATER FISHERIES
2014年
1期
73-77
,共5页
陈红莲%宋光同%何吉祥%侯冠军%王永杰
陳紅蓮%宋光同%何吉祥%侯冠軍%王永傑
진홍련%송광동%하길상%후관군%왕영걸
克氏原螯虾(Procambarus clarkii)%弗氏柠檬酸杆菌(Citrobacter freundii)%分离鉴定%16S rRNA基因%gyrB基因%药敏试验
剋氏原螯蝦(Procambarus clarkii)%弗氏檸檬痠桿菌(Citrobacter freundii)%分離鑒定%16S rRNA基因%gyrB基因%藥敏試驗
극씨원오하(Procambarus clarkii)%불씨저몽산간균(Citrobacter freundii)%분리감정%16S rRNA기인%gyrB기인%약민시험
Procambarus clarkii%isolation and identification%Citrobacter freundii%16 S rRNA gene%gyrB gene%antibiot-ics susceptibility test
为确定引起养殖克氏原螯虾（ Procambarus clarkii）患病的病原种类及耐药情况，从患病克氏原螯虾肝胰腺取样进行常规细菌学检查，对分离株进行回归感染试验、形态观察、生理生化特性检测、16S rRNA和gyrB基因分析及药敏试验。结果显示：从患病克氏原螯虾肝胰腺分离到大量菌落形态和色泽一致的细菌，代表菌株X120523为弗氏柠檬酸杆菌（ Citrobacter freundii），具有较强致病性，对20种抗生素中的喹诺酮类和氨基糖苷类抗生素敏感。
為確定引起養殖剋氏原螯蝦（ Procambarus clarkii）患病的病原種類及耐藥情況，從患病剋氏原螯蝦肝胰腺取樣進行常規細菌學檢查，對分離株進行迴歸感染試驗、形態觀察、生理生化特性檢測、16S rRNA和gyrB基因分析及藥敏試驗。結果顯示：從患病剋氏原螯蝦肝胰腺分離到大量菌落形態和色澤一緻的細菌，代錶菌株X120523為弗氏檸檬痠桿菌（ Citrobacter freundii），具有較彊緻病性，對20種抗生素中的喹諾酮類和氨基糖苷類抗生素敏感。
위학정인기양식극씨원오하（ Procambarus clarkii）환병적병원충류급내약정황，종환병극씨원오하간이선취양진행상규세균학검사，대분리주진행회귀감염시험、형태관찰、생리생화특성검측、16S rRNA화gyrB기인분석급약민시험。결과현시：종환병극씨원오하간이선분리도대량균락형태화색택일치적세균，대표균주X120523위불씨저몽산간균（ Citrobacter freundii），구유교강치병성，대20충항생소중적규낙동류화안기당감류항생소민감。
This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia.Pathogenic bacteria from hepatopancreas of the diseased Procambarus clarkia were examined using conventional methods and isolated.The further tests and analysis of the isolated strain were developed , including the regression experi-ment to Procambarus clarkii, the morphology, physiological and biochemical characteristics , sequence analysis of their 16S rRNA and gyrB genes , and the susceptibility test to antibiotics.Large colonies with similar morphology and color were obtained.Strain X120523 was identified as Citrobacter freundii, proved to have strong pathogenicity , and susceptible to quinolones and aminoglycosides.
