CAJ | 학술논문

以马鹿（ Cervus elaphus）为试验动物，采用上浮法诱导马鹿精子体外获能，以钙离子载体（ A23187）诱导马鹿精子顶体反应，进而摸索钙离子载体（ A23187）诱发其顶体反应的适宜体系。结果表明：0．01～100．00μmol/L的A23187可提高获能马鹿精子的顶体反应率；其中适宜的A23187浓度为0．10μmol/L，反应的时间为5 min，顶体反应率为（74．00±2．62）％。 A23187能促进获能马鹿精子的顶体反应，但不能显著（P＞0．05）促进未获能马鹿精子的顶体反应。
이마록（ Cervus elaphus）위시험동물，채용상부법유도마록정자체외획능，이개리자재체（ A23187）유도마록정자정체반응，진이모색개리자재체（ A23187）유발기정체반응적괄의체계。결과표명：0．01～100．00μmol/L적A23187가제고획능마록정자적정체반응솔；기중괄의적A23187농도위0．10μmol/L，반응적시간위5 min，정체반응솔위（74．00±2．62）％。 A23187능촉진획능마록정자적정체반응，단불능현저（P＞0．05）촉진미획능마록정자적정체반응。
Wapiti (Cervus elaphus) sperm was capacitated in vitro by swimming-up and the acrosome reaction (AR) was in-duced by Calcium ionophore A23187 to choose an optimal A23187 system during AR.A23187 of 0.01-100 μmol/L can enhance the ratio of AR on the capacitated sperm and 0.1μmol/L A23187 can obtain the highest ratio of AR (74.00%± 2.62%) for 5-min treatment time.AR of capacitated sperm can, but the uncapacitated sperm cannot, be induced by A23187 in wapiti.