CAJ | 학술논문

以大花剪秋萝（ Lychnis fulgens Fisch．）种子为外植体进行不定芽诱导，探索大花剪秋萝的离体快繁技术，建立快繁体系。试验以MS为基本培养基，添加不同激素，研究组织培养的最佳条件。结果显示，6-BA（细胞分裂素）对不定芽诱导有极显著影响，植物激素KT、ZT不显著；6-BA与NAA（奈乙酸）按一定比例配比，均对大花剪秋萝增殖起到促进作用，作用效果极为显著。得出最适宜种子诱导无菌苗的培养基为MS，出苗率为86％；最佳诱导丛生芽的增殖培养基为：MS＋3．00 mg· L-16-BA＋0．03 mg· L-1 NAA＋35 g· L-1蔗糖，pH值为5．80，其平均增殖系数为5．7，且诱导的无菌苗健壮；最适宜生根培养基1/2MS＋1．0 mg· L-1 NAA，生根率高达88％以上，生根健壮且数量繁多。为提高试管苗的增殖率及避免玻璃化现象的发生，最佳继代周期为25 d。
이대화전추라（ Lychnis fulgens Fisch．）충자위외식체진행불정아유도，탐색대화전추라적리체쾌번기술，건립쾌번체계。시험이MS위기본배양기，첨가불동격소，연구조직배양적최가조건。결과현시，6-BA（세포분렬소）대불정아유도유겁현저영향，식물격소KT、ZT불현저；6-BA여NAA（내을산）안일정비례배비，균대대화전추라증식기도촉진작용，작용효과겁위현저。득출최괄의충자유도무균묘적배양기위MS，출묘솔위86％；최가유도총생아적증식배양기위：MS＋3．00 mg· L-16-BA＋0．03 mg· L-1 NAA＋35 g· L-1자당，pH치위5．80，기평균증식계수위5．7，차유도적무균묘건장；최괄의생근배양기1/2MS＋1．0 mg· L-1 NAA，생근솔고체88％이상，생근건장차수량번다。위제고시관묘적증식솔급피면파리화현상적발생，최가계대주기위25 d。
With Lychnis fulgens Fisch seeds as explants for adventitious bud, the experiment was conducted to explore L.fulgens Fisch in vitro rapid propagation technology and establish a system of rapid propagation .With MS as basic culturemedium by adding different hormones, the test was conducted to study the best condition of tissue culture.6-BA has significant effect on adventitious bud induction , but no significance on phytohormones of KT and ZT.The combination of 6-BA and naphthalene aceticacid ( NAA) can be used for propagation subculture of L.fulgens Fisch with significant effect.The best medium for seed induced aseptic seedling is MS, and the emergence rate is 86%.The optimum medium for inducing the shoot cluster is MS+3.0 mg· L-1 6-BA+0.03 mg· L-1 NAA, pH is 5.80 and the propagation coefficient is 5.7 with vigor-ous aseptic seedling.For rooting culture, 1/2MS medium with 1.0 mg· L-1 NAA is the best medium with more than 88%rooting rate and well developed roots.The best subculture period should be 25 days to enhance the proliferation rate of test-tube plantlets and avoid vitrification.