茶叶科学
茶葉科學
다협과학
2014年
1期
95-104
,共10页
刘威%叶乃兴%陈玉森%连玲丽%刘伟%金珊%赖建东%谢运海
劉威%葉迺興%陳玉森%連玲麗%劉偉%金珊%賴建東%謝運海
류위%협내흥%진옥삼%련령려%류위%금산%뢰건동%사운해
茶树%Colletotrichum fructicola%鉴定%系统发育%rDNA-ITS%序列分析
茶樹%Colletotrichum fructicola%鑒定%繫統髮育%rDNA-ITS%序列分析
다수%Colletotrichum fructicola%감정%계통발육%rDNA-ITS%서렬분석
tea plant(Camellia sinensis)%Colletotrichum fructicola%pathogen identification%phylogenetic analysis%rDNA-ITS%sequence analysis
从福建地区不同品种茶树上分离获得 ZHG、WSX、WHG、FTG、ZTG、AHD、WRG、SLH 等8株能侵染茶树的病原菌,采用形态学和 rDNA-ITS 序列分析相结合的方法对其进行鉴定。结果发现,SLH 菌株为胶孢炭疽菌Colletotrichum gloeosporioides,其余7个菌株均为Colletotrichum fructicola。C. fructicola为能侵染茶树的新记录种炭疽菌,将其 ITS 序列与寄主分别为油茶、茉莉和番石榴等木本植物的9株炭疽菌的 ITS序列进行聚类分析,结果显示,来自茶树的7个 C. fructicola 菌株种内存在碱基突变或缺失,且与寄主为茶树、油茶、茉莉等植物的胶孢炭疽菌C. gloeosporioides遗传距离较近。
從福建地區不同品種茶樹上分離穫得 ZHG、WSX、WHG、FTG、ZTG、AHD、WRG、SLH 等8株能侵染茶樹的病原菌,採用形態學和 rDNA-ITS 序列分析相結閤的方法對其進行鑒定。結果髮現,SLH 菌株為膠孢炭疽菌Colletotrichum gloeosporioides,其餘7箇菌株均為Colletotrichum fructicola。C. fructicola為能侵染茶樹的新記錄種炭疽菌,將其 ITS 序列與寄主分彆為油茶、茉莉和番石榴等木本植物的9株炭疽菌的 ITS序列進行聚類分析,結果顯示,來自茶樹的7箇 C. fructicola 菌株種內存在堿基突變或缺失,且與寄主為茶樹、油茶、茉莉等植物的膠孢炭疽菌C. gloeosporioides遺傳距離較近。
종복건지구불동품충다수상분리획득 ZHG、WSX、WHG、FTG、ZTG、AHD、WRG、SLH 등8주능침염다수적병원균,채용형태학화 rDNA-ITS 서렬분석상결합적방법대기진행감정。결과발현,SLH 균주위효포탄저균Colletotrichum gloeosporioides,기여7개균주균위Colletotrichum fructicola。C. fructicola위능침염다수적신기록충탄저균,장기 ITS 서렬여기주분별위유다、말리화번석류등목본식물적9주탄저균적 ITS서렬진행취류분석,결과현시,래자다수적7개 C. fructicola 균주충내존재감기돌변혹결실,차여기주위다수、유다、말리등식물적효포탄저균C. gloeosporioides유전거리교근。
Eight strains of anthracnose pathogen, ZHG, WSX, ZTG, WHG, FTG, AHD, WRG and SLH were isolated from different tea cultivars in Fujian Province. These strains were identified by using morphological method and rDNA-ITS sequence analysis. The results showed that SLH strain was identified as Colletotrichum gloeosporioides, while the other seven strains were Colletotrichum fructicola, which has never been discovered on tea plant. The ITS sequences of C. fructicola and those of nine strains of anthracnose pathogen from tea-oil tree, jasmine plant and papaya (psidium guajave), were got for a clustering analysis. The results showed that base mutations or deletions were existed in the seven strains from tea plant, and the seven strains of C. fructicola were geneticly close to the pathogen C. gloeosporioides, isolated from tea plant, oil-tea plant and jasmine plant.