茶叶科学
茶葉科學
다협과학
2014年
1期
71-78
,共8页
吕海鹏%钟秋生%施江%张悦%林智
呂海鵬%鐘鞦生%施江%張悅%林智
려해붕%종추생%시강%장열%림지
普洱茶%挥发性成分%顶空固相微萃取-气相色谱-质谱%指纹图谱
普洱茶%揮髮性成分%頂空固相微萃取-氣相色譜-質譜%指紋圖譜
보이다%휘발성성분%정공고상미췌취-기상색보-질보%지문도보
Pu-erh tea%volatile constituents%HS-SPME/GC-MS%fingerprint
采用顶空固相微萃取-气相色谱-质谱(HS-SPME/GC-MS)技术对普洱茶挥发性成分的指纹图谱进行了研究。结果表明,该技术可使普洱茶的挥发性成分得到良好的分离;通过对17个样品的 GC图谱的分析,共确定了26个色谱峰为普洱茶挥发性成分的特征指纹峰,建立了普洱茶挥发性成分的 GC-MS 指纹图谱,并对指纹图谱进行了相似度分析。该方法重复性好,挥发性成分中各成分分离较好,因此所建立的指纹图谱可作为普洱茶香气品质鉴定和评价的参考依据之一。
採用頂空固相微萃取-氣相色譜-質譜(HS-SPME/GC-MS)技術對普洱茶揮髮性成分的指紋圖譜進行瞭研究。結果錶明,該技術可使普洱茶的揮髮性成分得到良好的分離;通過對17箇樣品的 GC圖譜的分析,共確定瞭26箇色譜峰為普洱茶揮髮性成分的特徵指紋峰,建立瞭普洱茶揮髮性成分的 GC-MS 指紋圖譜,併對指紋圖譜進行瞭相似度分析。該方法重複性好,揮髮性成分中各成分分離較好,因此所建立的指紋圖譜可作為普洱茶香氣品質鑒定和評價的參攷依據之一。
채용정공고상미췌취-기상색보-질보(HS-SPME/GC-MS)기술대보이다휘발성성분적지문도보진행료연구。결과표명,해기술가사보이다적휘발성성분득도량호적분리;통과대17개양품적 GC도보적분석,공학정료26개색보봉위보이다휘발성성분적특정지문봉,건립료보이다휘발성성분적 GC-MS 지문도보,병대지문도보진행료상사도분석。해방법중복성호,휘발성성분중각성분분리교호,인차소건립적지문도보가작위보이다향기품질감정화평개적삼고의거지일。
The fingerprint on volatile constituents of Pu-erh tea was investigated using HS-SPME/GC-MS. Results showed that the volatile constituents of Pu-erh tea can be well separated using HS-SPME/GC-MS. Based on the analysis of GC chromatogram of 17 tea samples collected from Yunnan province, it was confirmed that 26 peaks in chromatograms can be selected as the fingerprint of peaks of volatile constituents, and it was established as the fingerprint chromatogram for Pu-erh tea. Moreover, the GC fingerprints were analyzed by similarity analysis. The method is of high degree of reproducibility and the resolving of each component is satisfactory, thus the established fingerprints could be used as one of the aroma quality control index for identification and quality assessment of Pu-erh tea.