CAJ | 학술논문

目的：探索BD Influx流式细胞仪分选细胞亚群的调试参数和最佳设定条件。方法：使用两种质控试剂Rainbow Beads和Accudrop Beads作为实验材料，对BD Influx流式细胞仪的针孔、液流、荧光通道、前向角散射光信号、液滴频率、振幅和液滴延迟等参数进行优化。结果：使用型号为100μm的喷嘴，在液流聚焦清楚、液柱打入废液收集管的正中央、荧光光斑进入针孔中央且最亮、前向角散射(FSC)光光斑进入针孔中央且最亮、断点最高、液流分叉呈稳定的亮点、振幅为5.20±1.00以及液滴延迟为35.20±2.00等最佳设定条件下，其细胞样本分选纯度可达99%，工作效率可达95%。结论：流式细胞仪分选参数条件的设定，可为获得高纯度、高活性的细胞提供快速调试方法。
목적：탐색BD Influx류식세포의분선세포아군적조시삼수화최가설정조건。방법：사용량충질공시제Rainbow Beads화Accudrop Beads작위실험재료，대BD Influx류식세포의적침공、액류、형광통도、전향각산사광신호、액적빈솔、진폭화액적연지등삼수진행우화。결과：사용형호위100μm적분취，재액류취초청초、액주타입폐액수집관적정중앙、형광광반진입침공중앙차최량、전향각산사(FSC)광광반진입침공중앙차최량、단점최고、액류분차정은정적량점、진폭위5.20±1.00이급액적연지위35.20±2.00등최가설정조건하，기세포양본분선순도가체99%，공작효솔가체95%。결론：류식세포의분선삼수조건적설정，가위획득고순도、고활성적세포제공쾌속조시방법。
Objective:To explore the optimal conditions and adjustment of sorting parameters for BD Influx flow cytometer. Methods: Using Rainbow Beads and Accudrop Beads as experimental materials, optimization of pinhole, stream, fluorescence channel, forward scatter signal, drop frequency, piezo amplitude and drop delay was conducted. Results: The optimal condition was as follows, using the 100 μm nozzle, the stream was centered on the pinholes and sharply focused, the stream was aimed at the drain, the brightest signal was in the center of the pinhole after optimizing the fluorescence channel, the brightest signal was in the center of the FSC pinhole after optimizing the forward scatter signal, the breakoff point was highest after adjusting the drop frequency, and the droplet shape remained constant. When the piezo amplitude was around 5.20±1.00 and the drop delay value was around 35.20±2.00, the adjustment efficiency achieved its optimal condition, with its purity at 99%and sorting efficiency at 95%. Conclusion:Rapid setting of optimal conditions and adjustment of sorting parameters for BD Influx flow cytometer guarantees the success of gathering cells of high purity and activity after cell sorting.