CAJ | 학술논문

目的：观察大鼠缺血性脑卒中后皮层中磷酸化EphrinB2的表达。方法将成年雄性Sprague-Dawley大鼠随机分为假手术组和模型组，每组12只。采用线栓法制作大鼠大脑中动脉阻塞(MCAO)缺血再灌注模型。利用Western blotting和免疫组化SP法检测两组大鼠脑皮层中磷酸化EphrinB2的表达。结果模型组大鼠大脑皮层的磷酸化EphrinB2表达量和阳性细胞数目均高于假手术组(P<0.05)，并且血管内皮有磷酸化EphrinB2的表达。结论大鼠缺血性脑卒中后EphrinB2信号通路被激活。
목적：관찰대서결혈성뇌졸중후피층중린산화EphrinB2적표체。방법장성년웅성Sprague-Dawley대서수궤분위가수술조화모형조，매조12지。채용선전법제작대서대뇌중동맥조새(MCAO)결혈재관주모형。이용Western blotting화면역조화SP법검측량조대서뇌피층중린산화EphrinB2적표체。결과모형조대서대뇌피층적린산화EphrinB2표체량화양성세포수목균고우가수술조(P<0.05)，병차혈관내피유린산화EphrinB2적표체。결론대서결혈성뇌졸중후EphrinB2신호통로피격활。
Objective To observe the expression of phosphorylated EphrinB2 in brain after focal cerebral ischemia/reperfusion in rats. Methods 24 male Sprague-Dawley rats were randomly divided into sham group (n=12) and model group (n=12). The model group was mod-eled as middle cerebral artery occlusion and reperfusion with nylon monofilament suture, and then was assessed with Longa's score. The ex-pression of phosphorylated EphrinB2 in cerebral cortex was detected with immunohistochemistry and Western blotting. Results The expres-sion of phosphorylated EphrinB2 and the number of positive cells were significantly higher in the sham group than in the control group (P<0.05). It existed in the vascular endothelium in cerebral cortex. Conclusion EphrinB2 signaling pathway is activated in ischemic stroke.