CAJ | 학술논문

目的：观察体外冲击波(ESW)对兔膝骨性关节炎软骨白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平的影响。方法将30只新西兰大白兔随机分为对照组、模型组和治疗组，每组各10只。模型组和治疗组采用改良伸直位固定6周，制备兔膝骨关节炎模型。治疗组造模后立即给予ESW治疗1次，能流密度0.1 mJ/mm2，冲击次数1000次。各组分别于治疗后4周处死，观察大体标本形态学变化，采用免疫组织化学法检测各组关节软骨中IL-1β和TNF-α的水平。结果治疗组IL-1β和TNF-α的表达较模型组明显下调(P<0.01)。结论 ESW能下调兔膝骨关节炎软骨细胞IL-1β、TNF-α的表达。
목적：관찰체외충격파(ESW)대토슬골성관절염연골백세포개소-1β(IL-1β)화종류배사인자-α(TNF-α)수평적영향。방법장30지신서란대백토수궤분위대조조、모형조화치료조，매조각10지。모형조화치료조채용개량신직위고정6주，제비토슬골관절염모형。치료조조모후립즉급여ESW치료1차，능류밀도0.1 mJ/mm2，충격차수1000차。각조분별우치료후4주처사，관찰대체표본형태학변화，채용면역조직화학법검측각조관절연골중IL-1β화TNF-α적수평。결과치료조IL-1β화TNF-α적표체교모형조명현하조(P<0.01)。결론 ESW능하조토슬골관절염연골세포IL-1β、TNF-α적표체。
Objective To explore the effect of extracorporeal shock wave (ESW) on expression of interleukin-1β(IL-1β) and tumor ne-crosis factor-α(TNF-α) in cartilage in rabbits with knee osteoarthritis. Methods 30 rabbits were randomly divided into control group, model group and treatment group, with 10 rabbits in each group. Model of knee osteoarthritis was established with modified plaster cast in exten-sion position for 6 weeks except the control group, and the treatment group was treated with ESW (each 1000 impulse, the energy flux densi-ty of 0.1 mJ/mm2). The rabbits were sacrificed respectively 4 weeks after ESW treatment, the general and histological changes of articular cartilage were examined and immunohistochemical staining was used to observe the expression of IL-1βand TNF-αin cartilage. Results The expression of IL-1βand TNF-αwere significantly lower in the treatment group than in the model group (P<0.01). Conclusion ESW can down-regulate the expression of IL-1βand TNF-αin chondrocytes with osteoarthritis.