CAJ | 학술논문

从窑湾甜油面菌块中，分别筛选得到一株霉菌菌株和一株酵母菌菌株。霉菌通过形态学鉴定，初步确定霉菌为毛霉科的爪哇毛霉（Mucor javanicus），命名为DCF-4。酵母菌通过形态特征、生理生化特征分析，初步确定为酒香酵母菌属（Brettanomyces），命名为XYS-2。对其淀粉酶酶学特性进行研究，结果表明：爪哇毛霉和酒香酵母菌产生淀粉酶酶活分别达到535．33 U，759．03 U。进一步对两株菌产生的淀粉酶酶学性质进行研究，结果显示爪哇毛霉DCF-4产生的淀粉酶（淀粉酶Ⅰ）最适温度为80℃，最适pH 为6．6，Ca2＋，Mg2＋，K＋对其酶活性有激活作用，而Fe2＋对其酶活有一定抑制作用；酒香酵母菌XYS-2产生的淀粉酶（淀粉酶Ⅱ）最适温度也为80℃，最适pH 为5．0，Ca2＋，Ba2＋，Na＋对其酶活性有激活作用，Cu2＋对其有明显酶活抑制作用，达到90％左右。有机溶剂对两种淀粉酶活性都具有较强的抑制作用。
종요만첨유면균괴중，분별사선득도일주매균균주화일주효모균균주。매균통과형태학감정，초보학정매균위모매과적조왜모매（Mucor javanicus），명명위DCF-4。효모균통과형태특정、생리생화특정분석，초보학정위주향효모균속（Brettanomyces），명명위XYS-2。대기정분매매학특성진행연구，결과표명：조왜모매화주향효모균산생정분매매활분별체도535．33 U，759．03 U。진일보대량주균산생적정분매매학성질진행연구，결과현시조왜모매DCF-4산생적정분매（정분매Ⅰ）최괄온도위80℃，최괄pH 위6．6，Ca2＋，Mg2＋，K＋대기매활성유격활작용，이Fe2＋대기매활유일정억제작용；주향효모균XYS-2산생적정분매（정분매Ⅱ）최괄온도야위80℃，최괄pH 위5．0，Ca2＋，Ba2＋，Na＋대기매활성유격활작용，Cu2＋대기유명현매활억제작용，체도90％좌우。유궤용제대량충정분매활성도구유교강적억제작용。
A mould strain and a yeast strain are screened from fermented flour of Yaowan sweet oil. The mould is identified through its morphological characteristics and it is identified as Mucor javani-cus,named as DCF-4.The yeast strain is identified through its morphological characteristics,physio-logical and biochemical characteristics and it is identified as Brettanomyces,named as XYS-2.The en-zyme properties produced by these two strains are studied.The amylase activity of the two strains re-spectively reaches 535.33 U and 759.03 U,and then the amylase properties are studied.The results show that the optimal temperature and pH value of amylase produced by Mucor javanicus (amylaseⅠ) respectively are 80 ℃and 6.6.Ca2+,Mg2+,K+ can promote amylase activity and Fe2+ can inhibit the amylase activity.The optimal temperature and pH value of amylase produced by Brettanomyces (am-ylaseⅡ)respectively are 80 ℃ and 5.0.Ca2+,Ba2+,Na+ can promote amylase activity.Moreover, Cu2+ can strongly inhibit amylase activity,which reaches about 90%.Organic solvent has strong inhi-bition to amylase activity of two strains.