中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2014年
2期
77-78,81
,共3页
中和试验%狐狸脑炎%幼狐%母源抗体
中和試驗%狐貍腦炎%幼狐%母源抗體
중화시험%호리뇌염%유호%모원항체
neutralization test%fox encephalitis%fox cubs%maternal antibody
本研究采用微量中和实验的方法,对30日龄、45日龄、60日龄幼狐的传染性脑炎母源抗体进行检测。结果显示,30日龄幼狐的传染性脑炎母源抗体水平较高,中和抗体平均效价为1:48.3;在45日龄断乳期该母源抗体平均中和抗体效价显著下降到1:18.3;在60日龄该母源抗体基本消失,多数中和抗体效价低于1:2.9。实验结果表明,45~50日龄对幼狐进行传染性脑炎疫苗首免,能避开免疫空白期和母源抗体干扰,获得较好免疫效果。
本研究採用微量中和實驗的方法,對30日齡、45日齡、60日齡幼狐的傳染性腦炎母源抗體進行檢測。結果顯示,30日齡幼狐的傳染性腦炎母源抗體水平較高,中和抗體平均效價為1:48.3;在45日齡斷乳期該母源抗體平均中和抗體效價顯著下降到1:18.3;在60日齡該母源抗體基本消失,多數中和抗體效價低于1:2.9。實驗結果錶明,45~50日齡對幼狐進行傳染性腦炎疫苗首免,能避開免疫空白期和母源抗體榦擾,穫得較好免疫效果。
본연구채용미량중화실험적방법,대30일령、45일령、60일령유호적전염성뇌염모원항체진행검측。결과현시,30일령유호적전염성뇌염모원항체수평교고,중화항체평균효개위1:48.3;재45일령단유기해모원항체평균중화항체효개현저하강도1:18.3;재60일령해모원항체기본소실,다수중화항체효개저우1:2.9。실험결과표명,45~50일령대유호진행전염성뇌염역묘수면,능피개면역공백기화모원항체간우,획득교호면역효과。
Maternal antibodies against fox encephalitis virus in fox cubs at ages of 30,45 and 60 days were detected by micro neutralization test. The results showed that the level of the maternal antibodies was higher at 30 day-old with an average SN titer of 1:48.3,it was reduced sharply to 1:18.3 at 45 day-old when weaning,and almost disappeared at 60 day-old as most of them were below 1:2.9,suggesting that good immunity could be obtained if the infectious fox encephalitis vaccine was inoculated for the first time between 45 and 50 day-old and the immune gap and the maternal antibody interference could be avoided as well.