CAJ | 학술논문

本研究对猫传染性腹膜炎病毒（FIPV）N蛋白的编码基因进行克隆和原核表达，并在纯化重组N蛋白的基础上，建立了FIPV抗体间接ELISA检测方法。研究结果显示，该重组纯化的N蛋白具有良好的抗原反应性，可用于FIPV阳性血清的筛查，为我国出入境检疫部门监控FIPV疫情提供技术支撑。
본연구대묘전염성복막염병독（FIPV）N단백적편마기인진행극륭화원핵표체，병재순화중조N단백적기출상，건립료FIPV항체간접ELISA검측방법。연구결과현시，해중조순화적N단백구유량호적항원반응성，가용우FIPV양성혈청적사사，위아국출입경검역부문감공FIPV역정제공기술지탱。
In this study,the gene of nucleocapsid protein(N protein)of feline infectious peritonitis virus(FIPV) was cloned and expressed in Escherichia coli. And an indirect ELISA method was established with the purified recombi-nant N protein. The results showed this recombinant N protein had perfect antigen reactivity of FIPV and could be used to screen FIPV positive serum,and the study was helpful to monitor the FIPV infection status.