CAJ | 학술논문

根据猪圆环病毒2型（PCV2）基因序列，设计1对特异性PCV2的鉴定引物，利用PCR方法对65份疑似PCV2感染的病料进行检测。PCR检测为阳性的病料经处理后，接种无PCV污染的Dulac细胞进行间接免疫荧光检测。对各分离毒株的全基因组进行PCR扩增、测序和基因型分析。结果表明：共分离鉴定21株PCV2，均属2b基因型，其中有16株为1C群，5株为1A/1B群。研究显示PCV2b 1C群已在江苏成为流行毒株。
근거저원배병독2형（PCV2）기인서렬，설계1대특이성PCV2적감정인물，이용PCR방법대65빈의사PCV2감염적병료진행검측。PCR검측위양성적병료경처리후，접충무PCV오염적Dulac세포진행간접면역형광검측。대각분리독주적전기인조진행PCR확증、측서화기인형분석。결과표명：공분리감정21주PCV2，균속2b기인형，기중유16주위1C군，5주위1A/1B군。연구현시PCV2b 1C군이재강소성위류행독주。
According to the genome sequences of porcine circovirus type 2(PCV2),one pair of primer was designed to detect PCV2 by PCR in 65 samples from the suspected cases of PCV2 infection. The positive samples were inoculated on to Dulac cells and subjected to indirect immunofluorescence assay. The genome sequences of the isolates were amplified with PCR ,sequenced,and gene typed. The results showed that a total of 21 PCV2 strains was isolated and identified,and all the isolates belonged to genotype 2b,of which 16 isolates were grouped into cluster 1C and 5 isolates were grouped into clusters 1A/1B. The data indicated that PCV2b cluster 1C was the prevailing strain in Jiangsu.