新疆医科大学学报
新疆醫科大學學報
신강의과대학학보
JOURNAL OF XINJIANG MEDICAL UNIVERSITY
2014年
2期
160-162
,共3页
高效液相色谱法%维药%蜀葵花%芦丁%紫云英苷%含量测定
高效液相色譜法%維藥%蜀葵花%蘆丁%紫雲英苷%含量測定
고효액상색보법%유약%촉규화%호정%자운영감%함량측정
HPLC%Uyghur Medicine%Althaea rosea (L )Gavan%rutin%astragalin%assay
目的：建立高效液相色谱法（HPLC 法）测定维药蜀葵花中芦丁和紫云英苷的含量。方法采用YMC-Pack ODS-A C18（4．6 mm×250 mm，5μm）色谱柱，以流动相 A（0．2％磷酸水溶液）和流动相 B（0．2％磷酸乙腈溶液）进行梯度洗脱，检测波长为360 nm，流速为0．6 mL/min，进样体积10μL。结果芦丁和紫云英苷分别在0．5～6．0μg/mL 和1．0～12．0μg/mL 浓度范围内呈良好线性关系；线性方程分别为 A ＝583．5C -0．623和 A ＝628．3C -0．27，相关系数 r 分别为0．9995和0．9991；芦丁和紫云英苷的平均加样回收率（n ＝6）分别为102．3％和100．8％，RSD 分别为0．89％和0．60％，蜀葵花样品中芦丁和紫云英苷的含量分别为0．30～0．32 mg/g 和0．43～0．46 mg/g。结论本方法简便、准确，重复性好，可用于维药蜀葵花中芦丁和紫云英苷的含量测定，为其质量控制提供参考。
目的：建立高效液相色譜法（HPLC 法）測定維藥蜀葵花中蘆丁和紫雲英苷的含量。方法採用YMC-Pack ODS-A C18（4．6 mm×250 mm，5μm）色譜柱，以流動相 A（0．2％燐痠水溶液）和流動相 B（0．2％燐痠乙腈溶液）進行梯度洗脫，檢測波長為360 nm，流速為0．6 mL/min，進樣體積10μL。結果蘆丁和紫雲英苷分彆在0．5～6．0μg/mL 和1．0～12．0μg/mL 濃度範圍內呈良好線性關繫；線性方程分彆為 A ＝583．5C -0．623和 A ＝628．3C -0．27，相關繫數 r 分彆為0．9995和0．9991；蘆丁和紫雲英苷的平均加樣迴收率（n ＝6）分彆為102．3％和100．8％，RSD 分彆為0．89％和0．60％，蜀葵花樣品中蘆丁和紫雲英苷的含量分彆為0．30～0．32 mg/g 和0．43～0．46 mg/g。結論本方法簡便、準確，重複性好，可用于維藥蜀葵花中蘆丁和紫雲英苷的含量測定，為其質量控製提供參攷。
목적：건립고효액상색보법（HPLC 법）측정유약촉규화중호정화자운영감적함량。방법채용YMC-Pack ODS-A C18（4．6 mm×250 mm，5μm）색보주，이류동상 A（0．2％린산수용액）화류동상 B（0．2％린산을정용액）진행제도세탈，검측파장위360 nm，류속위0．6 mL/min，진양체적10μL。결과호정화자운영감분별재0．5～6．0μg/mL 화1．0～12．0μg/mL 농도범위내정량호선성관계；선성방정분별위 A ＝583．5C -0．623화 A ＝628．3C -0．27，상관계수 r 분별위0．9995화0．9991；호정화자운영감적평균가양회수솔（n ＝6）분별위102．3％화100．8％，RSD 분별위0．89％화0．60％，촉규화양품중호정화자운영감적함량분별위0．30～0．32 mg/g 화0．43～0．46 mg/g。결론본방법간편、준학，중복성호，가용우유약촉규화중호정화자운영감적함량측정，위기질량공제제공삼고。
Objective HPLC method was used to determinate the content of rutin and astragalin inAlthaea rosea (L .)Gavan from Uyghur Medicine.Methods Analysis was carried out on an YMC-Pack ODS-A C18 (4.6 mm×250 mm,5 μm)column with 0.2% phosphoric acid in water 0.2% phosphoric acid in acetonitrile as mobile phase in gradient elution.The detective wavelength was 360 nm,flow rate was 0.6 mL/min and injec-tion volume was 10 μL.Results The linear ranges of rutin and astragalin were 0.5-6.0 μg/mL and 1.0-12.0 μg/mL and the linear equation were A =583.5C -0.623 (r =0.999 5)and A =628.3C - 0.270 (r =0.999 1)respectively.The average recoveries were 102.3% and 100.8% with RSD of 0.89% and 0.60% (n=6)respectively.The content of rutin and astragalin in Althaea rosea (L .)Gavan were 0.30-0.32 mg/g (n =3)and 0.43~0.46 mg/g (n =3)respectively.Conclusion The results show that the method is sim-ple,accurate and repeatable,and can be used for the quantitative analysis of rutin and astragalin inAlthaea rosea (L.)Gavan from Uyghur Medicine and can be used as a referent for its quality control.
