CAJ | 학술논문

目的：探讨 p53抗体在维吾尔族（维族）、汉族非小细胞肺癌（Non-small-cell carcinoma，NSCLC）患者血清中的表达及其临床意义。方法选择诊断为非小细胞肺癌且未经放化疗、手术治疗的患者50例（病例组），其中维族、汉族患者各25例，另选取维族与汉族健康体检者（对照组）各25例。采用酶联免疫吸附试验（ELISA）检测血清中 p53抗体含量，采用全自动免疫化学发光仪检测血清常用肿瘤标志物含量，并进行比较分析。结果维族病例组 p53抗体含量高于对照组，差异有统计学意义（P ＜0．01），且抗体表达与其分化程度、TNM 分期密切相关（P ＜0．05）；汉族病例组 p53抗体含量高于对照组，差异有统计学意义（P ＜0．01），抗体表达与其分化程度、TNM 分期密切相关（P ＜0．05）；维族病例组不同病理分型 p53抗体含量比较差异有统计学意义（P ＜0．05）；但两民族病例组和对照组 p53抗体含量比较差异均无统计学意义（P ＞0．05），病例组在性别、年龄、分化程度、TNM 分期以及病理分型上均无相关性（P ＞0．05），并且两民族患者血清 p53含量与其各自血清中肿瘤标志物含量差异无统计学意义（P ＞0．05）。结论维、汉两民族非小细胞肺癌患者血清中的 p53抗体的表达情况基本一致，临床上可以考虑给予维、汉非小细胞肺癌患者相同或者相似的与 p53相关的诊断及治疗措施，同样可以根据相同或相似的 p53抗体指标来判断非小细胞肺癌患者的预后。
목적：탐토 p53항체재유오이족（유족）、한족비소세포폐암（Non-small-cell carcinoma，NSCLC）환자혈청중적표체급기림상의의。방법선택진단위비소세포폐암차미경방화료、수술치료적환자50례（병례조），기중유족、한족환자각25례，령선취유족여한족건강체검자（대조조）각25례。채용매련면역흡부시험（ELISA）검측혈청중 p53항체함량，채용전자동면역화학발광의검측혈청상용종류표지물함량，병진행비교분석。결과유족병례조 p53항체함량고우대조조，차이유통계학의의（P ＜0．01），차항체표체여기분화정도、TNM 분기밀절상관（P ＜0．05）；한족병례조 p53항체함량고우대조조，차이유통계학의의（P ＜0．01），항체표체여기분화정도、TNM 분기밀절상관（P ＜0．05）；유족병례조불동병리분형 p53항체함량비교차이유통계학의의（P ＜0．05）；단량민족병례조화대조조 p53항체함량비교차이균무통계학의의（P ＞0．05），병례조재성별、년령、분화정도、TNM 분기이급병리분형상균무상관성（P ＞0．05），병차량민족환자혈청 p53함량여기각자혈청중종류표지물함량차이무통계학의의（P ＞0．05）。결론유、한량민족비소세포폐암환자혈청중적 p53항체적표체정황기본일치，림상상가이고필급여유、한비소세포폐암환자상동혹자상사적여 p53상관적진단급치료조시，동양가이근거상동혹상사적 p53항체지표래판단비소세포폐암환자적예후。
Objective To detect the expression of p53 antibody in the Uyghur,Han non-small cell lung cancer (Non-small-cell carcinoma,NSCLC)patients with serum,and analyze its clinical significance. Methods 50 non-small cell lung cancer patients were picked out as case group,including 25 Uyghur and Han patients respectively,who were not given chemotherapy treatment.Another 50 healthy people were picked out as control groups,also including 25 Uyghur and Han patients respectively.The enzyme-linked immunosorbent assay (ELISA)was used to detect the amount of p53 antibody and automatic chemilumi-nescence immunoassay was used serum to detect serum tumor markers levels.And then comparison and a-nalysis were made.Results p53 antibody results in both Uyghur and Han case groups were higher than those in control groups and the differences were statistically significant (P <0.01).Antibody expressions were closely related to the degree of differentiation and TNM stage,which was statistically significant (P<0.05);p53 antibody results showed no statistical significance between Uyghur and Han case groups and control groups.Gender,age,degree of differentiation,TNM stage and pathological type in two case groups showed no correlation (P >0.05),and the two peoples and their respective levels of serum p53 tumor markers in serum levels were not correlated (P >0.05).Conclusion The serum p53 antibody ex-pressions in Uyghur and Han ethnic non-small cell lung cancer patients are basically the same.Uyghur and Han non-small cell lung cancer patients can be give the same or similar diagnosis and treatment,the prog-nostic judgment can also be the same.