CAJ | 학술논문

背景：血管紧张素转换酶作为肾素-血管紧张素系统的关键酶，通过影响P物质降解等机制参与阿尔茨海默病的发生与发展。 　　目的：分析血管紧张素转换酶基因多态性与阿尔茨海默病的关系，探讨性别、高血压病对两者关系的影响。 　　方法：纳入96例阿尔茨海默病患者，102例年龄、性别等具有可比性的正常人作为对照组。采集血液，抗凝后进行DNA抽提，用PCR反应进行扩增，20 g/L琼脂糖凝胶电泳，紫外光下观察结果，分别计算II、DD型纯合子及ID杂合子基因型及基因型频率。在此基础上，综合分析收集的相关临床资料，进行性别及血压分层分析。 　　结果与结论：阿尔茨海默病组与对照组血管紧张素转换酶基因型和等位基因频率差异有显著性意义，伴有高血压的阿尔茨海默病患者与对照组基因型和等位基因频率差异有显著性意义；不同性别分层及血压正常的阿尔茨海默病组与对照组基因型和等位基因频率差异无显著性意义。提示血管紧张素转换酶基因多态性与阿尔茨海默病的发病有关，其中 II 基因型可能是阿尔茨海默病发病的危险因素，血管紧张素转换酶基因型可能是阿尔茨海默病伴高血压病的危险因素。
배경：혈관긴장소전환매작위신소-혈관긴장소계통적관건매，통과영향P물질강해등궤제삼여아이자해묵병적발생여발전。 　　목적：분석혈관긴장소전환매기인다태성여아이자해묵병적관계，탐토성별、고혈압병대량자관계적영향。 　　방법：납입96례아이자해묵병환자，102례년령、성별등구유가비성적정상인작위대조조。채집혈액，항응후진행DNA추제，용PCR반응진행확증，20 g/L경지당응효전영，자외광하관찰결과，분별계산II、DD형순합자급ID잡합자기인형급기인형빈솔。재차기출상，종합분석수집적상관림상자료，진행성별급혈압분층분석。 　　결과여결론：아이자해묵병조여대조조혈관긴장소전환매기인형화등위기인빈솔차이유현저성의의，반유고혈압적아이자해묵병환자여대조조기인형화등위기인빈솔차이유현저성의의；불동성별분층급혈압정상적아이자해묵병조여대조조기인형화등위기인빈솔차이무현저성의의。제시혈관긴장소전환매기인다태성여아이자해묵병적발병유관，기중 II 기인형가능시아이자해묵병발병적위험인소，혈관긴장소전환매기인형가능시아이자해묵병반고혈압병적위험인소。
BACKGROUND:Angiotensin-converting enzyme as a key enzyme of the renin-angiotensin system, through the degradation effects of substance P mechanism, is involved in the occurrence and development of Alzheimer’s disease. OBJECTIVE:To research the relationship between angiotensin-converting enzyme gene polymorphism and Alzheimer’s disease in Jiamusi region, as wel as the effect of gender and hypertension on the relationship. METHODS:This case-control study included 96 Alzheimer’s disease patients. Another 102 subjects served as controls coming from the same area and in the same environmental condition. DNA segments were amplified using PCR in 20 g/L agarose gel electrophoresis and observed under ultraviolet lamp. II, ID, DD genotypes and genotype frequencies were calculated for statistical analysis. On this basis, according to clinical data col ected, we investigated association of Alzheimer’s disease with hypertension and gender. RESULTS AND CONCLUSION:There was significant difference between Alzheimer’s disease patients and controls in angiotensin-converting enzyme genotypes and al ele frequency. There was statistical y significant difference between Alzheimer’s patients with hypertension and controls in angiotensin-converting enzyme genotypes and al ele frequency. There was no statistical difference between Alzheimer’s disease patients with different genders and controls in angiotensin-converting enzyme genotypes and al ele frequency. These findings indicate that there are some relationships between angiotensin-converting enzyme polymorphism and Alzheimer’s disease. II genotype is a risk factor for Alzheimer’s disease, angiotensin-converting enzyme II genotype is a risk factor for Alzheimer’s disease with hypertension.