CAJ | 학술논문

背景：运动促进冠状动脉侧支循环生成涉及众多的促血管生成相关因子，单纯对一个因子的研究很难明确侧支循环生成的信号通路和传导途径，许多促血管生长因子都与激肽释放酶-激肽系统相关，而运动对该系统的影响目前未见报道。 　　目的：观察运动诱导对心肌梗死大鼠缓激肽表达的影响。 　　方法：健康Wistar大鼠30只，随机分为对照组、心肌梗死组及运动组。对照组只开胸，缝扎点穿线，不进行冠状动脉结扎；其余2组制备心肌梗死模型。运动组在成功造模后给予跑台运动，30 min/d，运动4周。实验终点时取血以ELISA法检测大鼠血清缓激肽水平，采用左心房注射微球法取大鼠心肌组织测定相对血流量。 　　结果与结论：实验结束时运动组缓激肽水平显著高于心肌梗死组(P <0.001)，心肌梗死组缓激肽水平显著高于对照组(P<0.05)。心肌相对血流量实验结束时心肌梗死组、运动组均显著高于同组实验开始前(P<0.05， P<0.001)，实验结束时运动组心肌相对血流量显著高于心肌梗死组(P<0.01)。各组大鼠血清缓激肽含量与心肌相对血流量存在显著相关性。提示运动可以刺激缓激肽表达水平显著升高，使心肌血流量明显增加，说明激肽释放酶激肽系统在运动诱导的血管新生中发挥作用。
배경：운동촉진관상동맥측지순배생성섭급음다적촉혈관생성상관인자，단순대일개인자적연구흔난명학측지순배생성적신호통로화전도도경，허다촉혈관생장인자도여격태석방매-격태계통상관，이운동대해계통적영향목전미견보도。 　　목적：관찰운동유도대심기경사대서완격태표체적영향。 　　방법：건강Wistar대서30지，수궤분위대조조、심기경사조급운동조。대조조지개흉，봉찰점천선，불진행관상동맥결찰；기여2조제비심기경사모형。운동조재성공조모후급여포태운동，30 min/d，운동4주。실험종점시취혈이ELISA법검측대서혈청완격태수평，채용좌심방주사미구법취대서심기조직측정상대혈류량。 　　결과여결론：실험결속시운동조완격태수평현저고우심기경사조(P <0.001)，심기경사조완격태수평현저고우대조조(P<0.05)。심기상대혈류량실험결속시심기경사조、운동조균현저고우동조실험개시전(P<0.05， P<0.001)，실험결속시운동조심기상대혈류량현저고우심기경사조(P<0.01)。각조대서혈청완격태함량여심기상대혈류량존재현저상관성。제시운동가이자격완격태표체수평현저승고，사심기혈류량명현증가，설명격태석방매격태계통재운동유도적혈관신생중발휘작용。
BACKGROUND:Exercise can elicit generation of coronary col ateral circulation through numerous pro-angiogenic growth factors. It is not enough via a single factor to clearly definite signaling pathways for the generation of col ateral circulation. Many pro-angiogenic growth factors are involved in kal ikrein-kinin system, and there is no report about exercise effects on this system. OBJECTIVE:To study effects of exercise training on bradykinin expression in rats with myocardial infarction. METHODS:Thirty Wistar rats were randomly assigned to control group, myocardial infarction group and exercises training group. Rats in the control group were subjected to thoracotomy with no coronary artery ligation. Rats in the other two groups were modeled. In the exercise training group, 3 days after myocardial infarction, rats were subjected to 30-minute exercise training on treadmil , once a day for 4 weeks. At the terminal of the experiment, blood samples were obtained to analyze bradykinin expression by enzyme linked immunosorbent assay, and the myocardium was sampled to analyze relative blood flow by microsphere method. RESULTS AND CONCLUSION:After exercise training, bradykinin level in the exercise training group was significantly higher than that in the myocardial infarction group (P<0.001). At the end of experiment, the relative blood flows of the exercise training and myocardial infarction groups were both increased compared with before experiment (P<0.05, P<0.001). At the end of experiment, the relative blood flow of the exercise training group was significantly higher than that of the myocardial infarction group (P<0.01). In al the three groups, bradykinin level was significantly correlated with the relative blood flow in the myocardium. These findings indicate exercises training can improve bradykinin expression apparently and increase blood flow in the myocardium, which il ustrates that the kal ikrein-kinin system plays an important role in exercise induced angiogenesis.