CAJ | 학술논문

背景：线栓法是制作局灶性脑缺血再灌注模型的常用方法，防治大出血和顺利插线是其造模成功的关键和研究热点。 　　目的：对Longa线栓法进行改良，以期有效防治术中大出血和提高造模成功率。 　　方法：制备模型时颈外动脉两断端除了手术线结扎外，线结外端用电刀夹闭；插线时借助弯镊的力量使鱼线沿血管前内侧壁(翼腭动脉分叉处颈内动脉血管的方向)前行。术后进行大鼠行为评分，计算脑梗死体积和光镜下观察组织病理学变化。 　　结果与结论：电刀夹闭后的血管从横切面上的“圆”形变成“一”字形，线结不易脱落，有效防治术中大出血。借助弯镊的力量，可使插线成功率明显提高。模型大鼠脑梗死体积均明显增加，脑组织病理损伤加重。结果可见改良线栓法制作 SD 大鼠局灶性脑缺血再灌注模型，可有效防止线结脱落，提高插线成功率，模型制备成功。
배경：선전법시제작국조성뇌결혈재관주모형적상용방법，방치대출혈화순리삽선시기조모성공적관건화연구열점。 　　목적：대Longa선전법진행개량，이기유효방치술중대출혈화제고조모성공솔。 　　방법：제비모형시경외동맥량단단제료수술선결찰외，선결외단용전도협폐；삽선시차조만섭적역량사어선연혈관전내측벽(익악동맥분차처경내동맥혈관적방향)전행。술후진행대서행위평분，계산뇌경사체적화광경하관찰조직병이학변화。 　　결과여결론：전도협폐후적혈관종횡절면상적“원”형변성“일”자형，선결불역탈락，유효방치술중대출혈。차조만섭적역량，가사삽선성공솔명현제고。모형대서뇌경사체적균명현증가，뇌조직병리손상가중。결과가견개량선전법제작 SD 대서국조성뇌결혈재관주모형，가유효방지선결탈락，제고삽선성공솔，모형제비성공。
BACKGROUND:During the progress of focal cerebral ischemia-reperfusion in rat with transient suture occlusion, bleeding combating and the line being plugged into the cerebral middle artery smoothly are important for successful modeling and research focus. OBJECTIVE:To improve Longa suture occlusion method for focal cerebral ischemia reperfusion model in Sprague-Dawley rats in order to establish a simple surgical procedure with low rate of intraoperative bleeding and high rate of successful models. METHODS:The two ends of the external carotid artery were clipped with electric knife, in addition to ligation of surgery lines intraoperatively;with the help of pincett, a fishing line was plugged into the carotid artery along the anteriomedialis artery wal (the direction of the internal carotid artery from the pterygopalatine arterial bifurcation). Postoperative behavior scores in rats, infarct volume calculation and histopathological evaluation under optical microscope were done. RESULTS AND CONCLUSION:Transverse sections of the external carotid artery were changed from a“O” shape to a“-”shape after being clipped with electric knife. Subsequently, line nodes were not easy to fal off any more, effectively preventing intraoperative bleeding. With the power of pincett, the rate of plugging the fishing line into artery was significantly increased. Rat’s neurological score and infarct volumes were significantly increased, and brain tissue pathological injury worsened. The modified suture occlusion method for focal cerebral ischemia reperfusion models effectively prevented line nodes off, successful y increased the rate of suture occlusion and models in Sprague-Dawley rats were developed successful y.