CAJ | 학술논문

背景：细胞周期素A2是调节细胞周期的关键因子，关于细胞周期素A2在乳鼠心肌中的表达及定位情况还不明确。 　　目的：探索细胞周期素A2在C57乳鼠中的表达定位及表达，检测心肌细胞中增殖相关蛋白随着细胞周期素A2的表达而呈现的表达趋势。 　　方法：C57乳鼠分别于出生后0，3，7，14，28 d处死，留取心肌组织，行Western blot检测心肌组织中细胞周期素A2、增殖细胞核抗原及磷酸组蛋白H3的表达情况。免疫组化检测细胞周期素A2在出生后乳鼠中的定位及增殖细胞核抗原在心肌组织中的表达。 　　结果与结论：C57乳鼠出生后细胞周期素A2表达水平逐渐下调，直至第14天基本消失(P=0.001)，且细胞周期素A2在乳鼠出生后0 d定位于细胞核中，在第14天时主要存在于细胞质中，至第28天时，基本消失。增殖细胞核抗原在出生后0 d表达最强，随后表达逐渐减弱。而反映有丝分裂期的特异性蛋白磷酸组蛋白H3随时间表达逐渐减少，且表达强度基本与细胞周期素A2表达减弱相一致。
배경：세포주기소A2시조절세포주기적관건인자，관우세포주기소A2재유서심기중적표체급정위정황환불명학。 　　목적：탐색세포주기소A2재C57유서중적표체정위급표체，검측심기세포중증식상관단백수착세포주기소A2적표체이정현적표체추세。 　　방법：C57유서분별우출생후0，3，7，14，28 d처사，류취심기조직，행Western blot검측심기조직중세포주기소A2、증식세포핵항원급린산조단백H3적표체정황。면역조화검측세포주기소A2재출생후유서중적정위급증식세포핵항원재심기조직중적표체。 　　결과여결론：C57유서출생후세포주기소A2표체수평축점하조，직지제14천기본소실(P=0.001)，차세포주기소A2재유서출생후0 d정위우세포핵중，재제14천시주요존재우세포질중，지제28천시，기본소실。증식세포핵항원재출생후0 d표체최강，수후표체축점감약。이반영유사분렬기적특이성단백린산조단백H3수시간표체축점감소，차표체강도기본여세포주기소A2표체감약상일치。
BACKGROUND:Cyclin A2 is a key regulator of cellcycle. Location and expression of cyclin A2 in neonatal mouse myocardium is not clear. OBJECTIVE:To observe the location and expression of cyclin A2 in neonatal mouse cardiomyocytes and its relationship with the exit of cardiomyocytes from cellcycle. METHODS:Neonatal mice were kil ed to take myocardial tissues at 0, 3, 7, 14 and 28 days after birth. Western blot were used to detect the expression of cyclin A2, proliferating cellnucleus antigen and Phospho-histone H3. Immunohistochemitry detection was used to detect the location of cyclin A2 and expression of proliferation cellnucleus antigen at different time after birth. RESULTS AND CONCLUSION:Western blot showed the decrease of cyclin A2 after birth til disappeared at day 4 (P=0.001). Cyclin A2 located mainly in the nucleus after birth and exported to the cytoplasm at day 14, and basical y disappeared at day 28. Proliferating cellnucleus antigen showed gradual y decreased tendency after birth. Mitosis specific marker, Phospho-histone H3, exhibited a gradual decrease after birth, which was consistent with cyclin A2 in expression intensity.