CAJ | 학술논문

背景：多项实验表明牙胚组织在不同的时间点有不同的基因发挥作用，共同促进牙胚发育。 　　目的：观察牙本质基质蛋白1、成釉蛋白、Ⅰ型胶原蛋白和同源异型盒基因1在大鼠牙胚细胞体外培养的不同时间的表达。 　　方法：对体外培养后第1，3，6天的牙胚细胞提取RNA，反转录后采用实时定量PCR的方法检测牙本质基质蛋白1、成釉蛋白、Ⅰ型胶原蛋白和同源异型盒基因1mRNA相对表达水平的变化。 　　结果与结论：牙胚细胞中牙本质基质蛋白1、成釉蛋白和Ⅰ型胶原蛋白mRNA的表达随培养时间的延长而增加，在培养3 d时达到峰值(P<0.05)，而同源异型盒基因1 mRNA的表达随培养时间的延长而下降(P<0.05)。
배경：다항실험표명아배조직재불동적시간점유불동적기인발휘작용，공동촉진아배발육。 　　목적：관찰아본질기질단백1、성유단백、Ⅰ형효원단백화동원이형합기인1재대서아배세포체외배양적불동시간적표체。 　　방법：대체외배양후제1，3，6천적아배세포제취RNA，반전록후채용실시정량PCR적방법검측아본질기질단백1、성유단백、Ⅰ형효원단백화동원이형합기인1mRNA상대표체수평적변화。 　　결과여결론：아배세포중아본질기질단백1、성유단백화Ⅰ형효원단백mRNA적표체수배양시간적연장이증가，재배양3 d시체도봉치(P<0.05)，이동원이형합기인1 mRNA적표체수배양시간적연장이하강(P<0.05)。
BACKGROUND:Some studies have indicated that different genes in tooth germ tissue play a role at different time, contributing to tooth germ development. OBJECTIVE:To observe the expressions of dentin matrix protein 1, enamel protein, col agen I and homeobox gene 1 at different stage of in vitro culture of tooth germ cells. METHODS:RNA from tooth germ cells was extracted at days 1, 3, 6 of in vitro culture. After reverse transcription, real-time quantitative PCR detection was adopted to measure relative expression of dentin matrix protein 1, enamel protein, col agen I and homeobox gene 1 mRNA. RESULTS AND CONCLUSION:Dentin matrix protein 1, enamel protein, and col agen I mRNA expressions increased with culture time, and reached the peak at day 3 (P<0.05), whereas homeobox gene 1 mRNA decreased with culture time (P<0.05).