CAJ | 학술논문

背景：早期受损的软骨细胞在体外培养时容易产生去分化，表型不稳，常需添加一定的生长因子。 　　目的：观察胰岛素样生长因子1对成年兔创伤性关节炎早期关节软骨细胞体外增殖的促进作用。 　　方法：采用改良Hulth法制备成兔创伤性关节炎模型，造模成功后无菌条件下片状切取股骨远端及胫骨近端，用消化培养法培养软骨细胞。将软骨细胞随机分为2组，对照组加入含体积分数10%胎牛血清因子的DMEM培养液培养；实验组在对照组的基础上加入100μg/L的胰岛素样生长因子1。通过细胞形态学、细胞计数、细胞活性检测胰岛素样生长因子1对创伤性关节炎关节软骨细胞增殖的影响。 　　结果与结论：成功培养出早期创伤性关节炎兔软骨细胞，细胞多数为小细胞，形态有小梭形、小圆形及小多边形。苏木精-伊红染色显示实验组细胞数量多于对照组，MTT 实验证实实验组细胞的吸光度值大于对照组(P<0.01)。结果提示，胰岛素样生长因子1能促进早期创伤性关节炎模型兔软骨细胞的体外增殖。
배경：조기수손적연골세포재체외배양시용역산생거분화，표형불은，상수첨가일정적생장인자。 　　목적：관찰이도소양생장인자1대성년토창상성관절염조기관절연골세포체외증식적촉진작용。 　　방법：채용개량Hulth법제비성토창상성관절염모형，조모성공후무균조건하편상절취고골원단급경골근단，용소화배양법배양연골세포。장연골세포수궤분위2조，대조조가입함체적분수10%태우혈청인자적DMEM배양액배양；실험조재대조조적기출상가입100μg/L적이도소양생장인자1。통과세포형태학、세포계수、세포활성검측이도소양생장인자1대창상성관절염관절연골세포증식적영향。 　　결과여결론：성공배양출조기창상성관절염토연골세포，세포다수위소세포，형태유소사형、소원형급소다변형。소목정-이홍염색현시실험조세포수량다우대조조，MTT 실험증실실험조세포적흡광도치대우대조조(P<0.01)。결과제시，이도소양생장인자1능촉진조기창상성관절염모형토연골세포적체외증식。
BACKGROUND:The early damaged chondrocytes are susceptible to de-differentiate and exert unstable phenotype during the in vitro culture, thus needing some growth factors. OBJECTIVE:To observe the promotion effect of insulin-like growth factor 1 on the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis. METHODS:Traumatic arthritis models of adult rabbits were established by using the modified Hulth method. After the models were successful y established, the distal femur and proximal tibia were harvested under sterile conditions, the chondrocytes were cultured. The cultured cells were divided into two groups:control group was cultured with Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum, while experimental group was cultured with Dulbecco's modified Eagle’s medium containing 100μg/L insulin-like growth factor 1. The effect of insulin-like growth factor 1 on the proliferation of chondrocytes in adult rabbits with traumatic arthritis was determined through the cytomorphology, cellcounting, and cellactivity. RESULTS AND CONCLUSION:The chondrocytes in adult rabbits with traumatic arthritis were successful y cultured, the majority of cells were mini-cells, presenting smal fusiform, round or polygonal shape. Hematoxylin-eosin staining showed that the number of cells in experimental group was higher than that in control group. MTT assay found that the absorbance of cells in experimental group was greater than that in control group (P<0.01). Our findings indicate that, insulin-like growth factor 1 can promote the in vitro proliferation of chondrocytes in adult rabbits with traumatic arthritis.