中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
2期
177-182
,共6页
汪宗保%徐亚林%廖威明%陆晴友%陈朝晖%吴向阳%孙旭%段文秀%鲍永军%代月兰
汪宗保%徐亞林%廖威明%陸晴友%陳朝暉%吳嚮暘%孫旭%段文秀%鮑永軍%代月蘭
왕종보%서아림%료위명%륙청우%진조휘%오향양%손욱%단문수%포영군%대월란
组织构建%软骨组织工程%骨关节炎%关节软骨%木瓜蛋白酶%半胱氨酸%电镜%软骨表面
組織構建%軟骨組織工程%骨關節炎%關節軟骨%木瓜蛋白酶%半胱氨痠%電鏡%軟骨錶麵
조직구건%연골조직공정%골관절염%관절연골%목과단백매%반광안산%전경%연골표면
osteoarthritis%papain%L-cysteine%cartilage,articular
背景:木瓜蛋白酶注射建立骨性关节炎动物模型是用于骨关节炎防治研究的常用方法之一。<br> 目的:观察木瓜蛋白酶和L-半胱氨酸混合注射诱导大鼠膝早期骨关节炎进程中扫描电镜下软骨表面形态学变化。<br> 方法:2%木瓜蛋白酶和0.03 mol/L左旋半胱氨酸按2∶1比例混匀,取0.15 mL注射至SD大鼠右膝关节腔诱导骨关节炎模型,左膝注射等量生理盐水为对照组,另取2只4膝不做处理为正常对照组,于注射后第2,4,6周后分别使用扫描电镜观察股骨内侧髁关节软骨表面形态学变化。<br> 结果与结论:正常和对照组可见表面分布较均匀的浅坑。木瓜蛋白酶和L-半胱氨酸混合注射2周后大鼠软骨表面出现凹凸不平,皱缩扭曲变形;4周表面变薄,局部出现小裂纹;6周出现深大裂纹,软骨缺损。提示2%木瓜蛋白酶和0.03 mol/L左旋半胱氨酸混合注射诱导的早期骨关节炎模型的时间节点可以定在4-6周。
揹景:木瓜蛋白酶註射建立骨性關節炎動物模型是用于骨關節炎防治研究的常用方法之一。<br> 目的:觀察木瓜蛋白酶和L-半胱氨痠混閤註射誘導大鼠膝早期骨關節炎進程中掃描電鏡下軟骨錶麵形態學變化。<br> 方法:2%木瓜蛋白酶和0.03 mol/L左鏇半胱氨痠按2∶1比例混勻,取0.15 mL註射至SD大鼠右膝關節腔誘導骨關節炎模型,左膝註射等量生理鹽水為對照組,另取2隻4膝不做處理為正常對照組,于註射後第2,4,6週後分彆使用掃描電鏡觀察股骨內側髁關節軟骨錶麵形態學變化。<br> 結果與結論:正常和對照組可見錶麵分佈較均勻的淺坑。木瓜蛋白酶和L-半胱氨痠混閤註射2週後大鼠軟骨錶麵齣現凹凸不平,皺縮扭麯變形;4週錶麵變薄,跼部齣現小裂紋;6週齣現深大裂紋,軟骨缺損。提示2%木瓜蛋白酶和0.03 mol/L左鏇半胱氨痠混閤註射誘導的早期骨關節炎模型的時間節點可以定在4-6週。
배경:목과단백매주사건립골성관절염동물모형시용우골관절염방치연구적상용방법지일。<br> 목적:관찰목과단백매화L-반광안산혼합주사유도대서슬조기골관절염진정중소묘전경하연골표면형태학변화。<br> 방법:2%목과단백매화0.03 mol/L좌선반광안산안2∶1비례혼균,취0.15 mL주사지SD대서우슬관절강유도골관절염모형,좌슬주사등량생리염수위대조조,령취2지4슬불주처리위정상대조조,우주사후제2,4,6주후분별사용소묘전경관찰고골내측과관절연골표면형태학변화。<br> 결과여결론:정상화대조조가견표면분포교균균적천갱。목과단백매화L-반광안산혼합주사2주후대서연골표면출현요철불평,추축뉴곡변형;4주표면변박,국부출현소렬문;6주출현심대렬문,연골결손。제시2%목과단백매화0.03 mol/L좌선반광안산혼합주사유도적조기골관절염모형적시간절점가이정재4-6주。
BACKGROUND:Osteoarthritis animal models induced by papain are commonly used for studying osteoarthritis. <br> OBJECTIVE:To observe the morphology of articular cartilage surface during early osteoarthritis process in rats induced by papain and L-cysteine under scanning electron microscope. <br> METHODS:The right knees of Sprague-Dawley rats were given 0.15 mL mixed solution of 2%papain and 0.03 mol/L L-cysteine in the ratio of 2:1. The left knees were given injections of 0.15 mL 0.9%NaCl, as the control group. Another two rats (four knees) maintained untreated, as the normal control group. Al the rats were observed with scanning electron microscope at 2, 4, 6 weeks after injection, to explore the surface morphology of medial condyle of femur. <br> RESULTS AND CONCLUSION:The condyles of normal and control groups revealed numerous shal ow pits on the surface of articular cartilage. At 2 weeks after injection, the surfaces of condyles were unsmooth, presenting the shrinkage and distortion. The thinner articular cartilage and local tiny cracks were seen at 4 weeks. The deep and great cracks and cartilage defect were observed on the surface of cartilage at 6 weeks. The time of 4-6 weeks is a reference point for establishing early osteoarthritis model in rats through injections of 2%papain and 0.03 mol/L L-cysteine.