CAJ | 학술논문

目的：探讨LY294002对成骨肉瘤细胞体外侵袭迁徙的影响。方法不同浓度的LY294002作用U2-OS细胞24 h，检测细胞增殖情况；Wound healing 和Transwell invasion 实验检测细胞迁徙和侵袭情况。结果 LY294002可抑制U2-OS细胞增殖并呈剂量依赖；LY294002（40μM）处理细胞组细胞迁徙率为（37．97±2．49）％，阴性对照组细胞迁徙率为（83．78±2．84）％，两组间具有显著的统计学差异（ P＜0．01）；LY294002（40μM）处理细胞组穿膜细胞数显著低于阴性对照组穿膜细胞数〔分别为（36．1±5．0）个/视野和（89．1±6．4）个/视野〕（ P＜0．01）。结论 LY294002能有效抑制成骨肉瘤细胞U2-OS细胞体外迁徙，有望成为成骨肉瘤化学治疗药物。
목적：탐토LY294002대성골육류세포체외침습천사적영향。방법불동농도적LY294002작용U2-OS세포24 h，검측세포증식정황；Wound healing 화Transwell invasion 실험검측세포천사화침습정황。결과 LY294002가억제U2-OS세포증식병정제량의뢰；LY294002（40μM）처리세포조세포천사솔위（37．97±2．49）％，음성대조조세포천사솔위（83．78±2．84）％，량조간구유현저적통계학차이（ P＜0．01）；LY294002（40μM）처리세포조천막세포수현저저우음성대조조천막세포수〔분별위（36．1±5．0）개/시야화（89．1±6．4）개/시야〕（ P＜0．01）。결론 LY294002능유효억제성골육류세포U2-OS세포체외천사，유망성위성골육류화학치료약물。
Objective Objective To investigate the effect of LY 294002 on invasion and migration in vitro of osteosarco-ma cells.Methods Various concentrations of LY294002 were adopted to deal with human osteosarcoma cells U 2-OS for 24 h;The cell proliferation was detected by MTT assay;Wound healing and Transwell invasion assays were performed to evaluate the cell migration and invasion abilities .Results The LY294002 could inhibite U2-OS cell proliferation dramatically and the inhibi-tory effect was concentration-dependent .The cell migration rates in LY294002 management group and the negative group were (37.9 ±2.49)%and (83.78 ±2.84)%,respectively.There was statistical difference .The trans-membraned cells in LY294002 management group were significantly lower than those of the negative group (36.1 ±5.0/HP and 89.1 ±6.4/HP,respectively) (P<0.01).Conclusion LY294002 could efficiently inhibit migration and invasion in vitro of osteosarcoma cells U 2-OS,and it has the potential to become a molecular target for anti-osteosarcoma metastasis .