世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2014年
2期
221-223
,共3页
杨珊莉%江一静%陶静%陈立典
楊珊莉%江一靜%陶靜%陳立典
양산리%강일정%도정%진립전
脑缺血%再灌注损伤%电针%cAMP反应原件结合蛋白
腦缺血%再灌註損傷%電針%cAMP反應原件結閤蛋白
뇌결혈%재관주손상%전침%cAMP반응원건결합단백
Cerebral ischemia%Reperfusion injury%Electro-acupuncture%CREB
目的:探寻电针对缺血性脑卒中再灌注损害大鼠cAMP反应原件结合蛋白( cAMP-response Element Binding Protein , CREB)表达的影响。方法:将60只健康成年的SD大鼠驯养3 d随机分成假手术组、模型组以及电针组,每组各20只,使用线栓法大脑中动脉闭塞( Middle Cerebral Artery Occlusion ,MCAO)对模型组、电针组大鼠进行脑缺血模型制作,缺血2 h后进行再灌注,电针组大鼠接受电针干预,再灌注3 d后将大鼠断头取脑,进行2,3,5-氯化三苯基四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色以及图像软件分析观察脑梗死体积,免疫蛋白印迹法(Western blotting)检测CREB蛋白水平,聚合酶链反应( Reverse Transcription-polymerase Chain Reaction ,PCR)检测CREB的基因表达水平。结果:TTC及其图像软件分析表明,电针组的SD大鼠脑梗死面积明显小于模型组( P<0.01);电针组的SD大鼠CREB蛋白水平明显高于模型组( P<0.01);电针组的SD大鼠CREB基因水平明显高于模型组( P<0.01)。结论:电针改善脑缺血再灌注损伤可以通过上调CREB实现。
目的:探尋電針對缺血性腦卒中再灌註損害大鼠cAMP反應原件結閤蛋白( cAMP-response Element Binding Protein , CREB)錶達的影響。方法:將60隻健康成年的SD大鼠馴養3 d隨機分成假手術組、模型組以及電針組,每組各20隻,使用線栓法大腦中動脈閉塞( Middle Cerebral Artery Occlusion ,MCAO)對模型組、電針組大鼠進行腦缺血模型製作,缺血2 h後進行再灌註,電針組大鼠接受電針榦預,再灌註3 d後將大鼠斷頭取腦,進行2,3,5-氯化三苯基四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色以及圖像軟件分析觀察腦梗死體積,免疫蛋白印跡法(Western blotting)檢測CREB蛋白水平,聚閤酶鏈反應( Reverse Transcription-polymerase Chain Reaction ,PCR)檢測CREB的基因錶達水平。結果:TTC及其圖像軟件分析錶明,電針組的SD大鼠腦梗死麵積明顯小于模型組( P<0.01);電針組的SD大鼠CREB蛋白水平明顯高于模型組( P<0.01);電針組的SD大鼠CREB基因水平明顯高于模型組( P<0.01)。結論:電針改善腦缺血再灌註損傷可以通過上調CREB實現。
목적:탐심전침대결혈성뇌졸중재관주손해대서cAMP반응원건결합단백( cAMP-response Element Binding Protein , CREB)표체적영향。방법:장60지건강성년적SD대서순양3 d수궤분성가수술조、모형조이급전침조,매조각20지,사용선전법대뇌중동맥폐새( Middle Cerebral Artery Occlusion ,MCAO)대모형조、전침조대서진행뇌결혈모형제작,결혈2 h후진행재관주,전침조대서접수전침간예,재관주3 d후장대서단두취뇌,진행2,3,5-록화삼분기사담서(2,3,5-triphenyltetrazolium chloride,TTC)염색이급도상연건분석관찰뇌경사체적,면역단백인적법(Western blotting)검측CREB단백수평,취합매련반응( Reverse Transcription-polymerase Chain Reaction ,PCR)검측CREB적기인표체수평。결과:TTC급기도상연건분석표명,전침조적SD대서뇌경사면적명현소우모형조( P<0.01);전침조적SD대서CREB단백수평명현고우모형조( P<0.01);전침조적SD대서CREB기인수평명현고우모형조( P<0.01)。결론:전침개선뇌결혈재관주손상가이통과상조CREB실현。
Objective:To explore the impact of electro-acupuncture on the expression of CREB in the ischemia-reperfusion rats .Meth-ods:Sixty healthy adult SD rats which were domesticated for 3 days were randomly divided into the shame group , the model group and the electro-acupuncture group .There were 20 rats in each group .The rats of the model group and the electro-acupuncture group were given the line bolt method ( MCAO) to complete the brain ischemia model , and were reperfused 2 hours after ischemia .The electro-acu-puncture group adopted the intervention of electro-acupuncture .Three days after the reperfusion , all the rats were beheaded and taken brains.The cerebral infarction volume was observed through TTC staining and image analysis , and the protein expression of CREB were detected by protein imprinting method ( Western blotting ) and gene expression of CREB were tested by Real-Time PCR.Results:TTC and the image analysis showed that the cerebral infarction volume of electro -acupuncture group was significantly smaller than that of the model group (P<0.01);the CREB protein level in the electro-acupuncture group was obviously higher than that of the model group (P<0.01);the CREB gene level in the electro-acupuncture group was much higher than that of the model group (P<0.01).Conclu-sion:Electro-acupuncture can realize the improvement of the cerebral ischemia reperfusion injury by up -regulating CREB.