CAJ | 학술논문

目的：考察商陆皂苷甲Esculentoside A（EsA）对人肾小管上皮细胞（HK-2细胞）的毒性。方法：1）MTT法测细胞活力；2）检测细胞上清液LDH、细胞内SOD及MDA；3）采用倒置显微镜及电镜观察细胞形态学；4）采用Hoechst-PI染色及流式细胞仪观察细胞凋亡的情况。结果：1）EsA对肾细胞的活力有显著的抑制作用，其IC50为149.11μg· mL-1，且存在一定的时效和量效关系；2）EsA能升高肾细胞培养上清中的LDH，使肾细胞内SOD/MDA比值有所下降；3）EsA能使肾细胞及其超微结构发生变化，出现凋亡或坏死，有一定的量效关系。结论：大于100μg· mL-1浓度的商陆皂苷甲对HK-2细胞有一定的毒性，其毒性机制与细胞氧化应激、凋亡等相关。
목적：고찰상륙조감갑Esculentoside A（EsA）대인신소관상피세포（HK-2세포）적독성。방법：1）MTT법측세포활력；2）검측세포상청액LDH、세포내SOD급MDA；3）채용도치현미경급전경관찰세포형태학；4）채용Hoechst-PI염색급류식세포의관찰세포조망적정황。결과：1）EsA대신세포적활력유현저적억제작용，기IC50위149.11μg· mL-1，차존재일정적시효화량효관계；2）EsA능승고신세포배양상청중적LDH，사신세포내SOD/MDA비치유소하강；3）EsA능사신세포급기초미결구발생변화，출현조망혹배사，유일정적량효관계。결론：대우100μg· mL-1농도적상륙조감갑대HK-2세포유일정적독성，기독성궤제여세포양화응격、조망등상관。
Objective:To investigate the toxicity of Esculentoside A on HK-2 cells (Human renal tubular epithelial cells ).Methods:1) MTT assay was used to test the cell viability .2)The LDH in cells supernatant, SOD and MDA in the cells were detected .3)Morphologi-cal changes of the cells were observed through inverted phase contrast microscope and electron microscopy .4) Cell apoptosis was detec-ted by Hochest33342-PI dyeing and flow cytometry .Results:1) Esculentoside A could inhibit nephrocyte viability dependently on time and dose (IC50 was 149.11μg· mL-1).2)Esculentoside A could increase the activity of LDH in nephrocyte supernatant , reduce SOD activity and increase MDA content in nephrocytes .3 ) Esculentoside A could cause damage of nephrocyte structure , cell apoptosis and necrosis.Conclusion:It showed that large dose of Esculentoside A (more than 100 μg· mL-1) has toxicity on HK-2 cells and the mechanisms of toxicity may include cellular oxidative damage and cell apoptosis .