检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2014年
2期
139-142
,共4页
中性粒细胞明胶酶相关脂质运载蛋白%急性肾损伤%心脏手术
中性粒細胞明膠酶相關脂質運載蛋白%急性腎損傷%心髒手術
중성립세포명효매상관지질운재단백%급성신손상%심장수술
Neutrophil gelatinase-associated lipocalin%Acute kidney injury%Cardiac surgery
目的：探讨血清中性粒细胞明胶酶相关脂质运载蛋白（NGAL）在成人心脏术后急性肾损伤（AKI）早期诊断中的应用价值。方法收集成人心脏手术患者62例，分别在其术前及术后6、24、48 h采集血液标本。采用酶法检测血清肌酐（SCr），并以48 h内 SCr上升至≥26．5μmol/L作为AKI的诊断标准，其中23例发生AKI （AKI组）、39例未发生AKI（无AKI组）。应用乳胶增强免疫透射比浊法分别检测2组患者的血清NGAL和半胱氨酸蛋白酶抑制剂C（Cys C）水平。结果与术前比较，AKI组术后6、24、48 h血清NGAL水平均明显上升（P＜0．01）；血清Cys C水平在术后24、48 h明显上升（P＜0．01）。无AKI组术后6、24、48 h血清NGAL水平差异均无统计学意义（P＞0．05）；血清Cys C水平术后6、24 h差异无统计学意义（P＞0．05），术后48 h升高（P＜0．05）。结论血清NGAL水平在成人心脏术后发生AKI 6 h即明显上升，明显早于Cys C和SCr。血清NGAL可以作为早期诊断成人心脏术后AKI的标志物。
目的：探討血清中性粒細胞明膠酶相關脂質運載蛋白（NGAL）在成人心髒術後急性腎損傷（AKI）早期診斷中的應用價值。方法收集成人心髒手術患者62例，分彆在其術前及術後6、24、48 h採集血液標本。採用酶法檢測血清肌酐（SCr），併以48 h內 SCr上升至≥26．5μmol/L作為AKI的診斷標準，其中23例髮生AKI （AKI組）、39例未髮生AKI（無AKI組）。應用乳膠增彊免疫透射比濁法分彆檢測2組患者的血清NGAL和半胱氨痠蛋白酶抑製劑C（Cys C）水平。結果與術前比較，AKI組術後6、24、48 h血清NGAL水平均明顯上升（P＜0．01）；血清Cys C水平在術後24、48 h明顯上升（P＜0．01）。無AKI組術後6、24、48 h血清NGAL水平差異均無統計學意義（P＞0．05）；血清Cys C水平術後6、24 h差異無統計學意義（P＞0．05），術後48 h升高（P＜0．05）。結論血清NGAL水平在成人心髒術後髮生AKI 6 h即明顯上升，明顯早于Cys C和SCr。血清NGAL可以作為早期診斷成人心髒術後AKI的標誌物。
목적：탐토혈청중성립세포명효매상관지질운재단백（NGAL）재성인심장술후급성신손상（AKI）조기진단중적응용개치。방법수집성인심장수술환자62례，분별재기술전급술후6、24、48 h채집혈액표본。채용매법검측혈청기항（SCr），병이48 h내 SCr상승지≥26．5μmol/L작위AKI적진단표준，기중23례발생AKI （AKI조）、39례미발생AKI（무AKI조）。응용유효증강면역투사비탁법분별검측2조환자적혈청NGAL화반광안산단백매억제제C（Cys C）수평。결과여술전비교，AKI조술후6、24、48 h혈청NGAL수평균명현상승（P＜0．01）；혈청Cys C수평재술후24、48 h명현상승（P＜0．01）。무AKI조술후6、24、48 h혈청NGAL수평차이균무통계학의의（P＞0．05）；혈청Cys C수평술후6、24 h차이무통계학의의（P＞0．05），술후48 h승고（P＜0．05）。결론혈청NGAL수평재성인심장술후발생AKI 6 h즉명현상승，명현조우Cys C화SCr。혈청NGAL가이작위조기진단성인심장술후AKI적표지물。
Objective To investigate the early diagnosis significance of neutrophil gelatinase-associated lipocalin (NGAL)on acute kidney injury (AKI)in patients undergoing cardiac surgery.Methods Blood samples before and after surgery were collected from 62 patients undergoing cardiac surgery.The blood samples were collected before surgery and at 6,24 and 48 h after surgery respectively.The serum creatinine (SCr)was detected by enzymatic method.AKI was defined as diagnosis standard at SCr≥26.5 μmol/L within 48 h.There were 23 AKI patients (AKI group)and 39 patients without AKI (non AKI group).Serum NGAL and serum cystatin C(Cys C)were detected by latex-enhanced immunoturbidimetry.Results Serum NGAL increased significantly at 6,24,48 h after surgery(P<0.01 ),while serum Cys C increased at 24 and 48 h after surgery in AKI group (P<0.01 ).There was no statistical significance for NGAL in non AKI group before and at 6,24 and 48 h after surgery(P>0.05).There was no statistical significance for CysC in non AKI group before and at 6 and 24 h after surgery(P>0.05 ).However,Cys C in non AKI group at 48 h after surgery increased (P<0.05 ).Conclusions Serum NGAL at 6 h after cardiac surgery are predictive for AKI, which is much earlier than Cys C and SCr.Serum NGAL can be used as early markers for AKI after cardiac surgery.
