CAJ | 학술논문

目的：探讨鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌（MRSA）的分子流行病学特点，明确本地区MRSA基因型别及其分布规律。方法收集2009年1月至2011年8月临床分离的MRSA菌株54株。应用多重聚合酶链反应（PCR）对MRSA菌株进行葡萄球菌染色体mec（SCCmec）基因分型、杀白细胞毒素（PVL）毒力基因（pvl）和多位点序列分型（MLST）检测。结果 MRSA菌株的SCCmec Ⅰ～Ⅴ基因分型分别占0．00％、50．00％、46．30％、1．85％和1．85％；检测出1株pvl基因阳性；24株MRSA菌株MLST分型显示，ST23913株（54．17％），ST59株（37．50％），ST59和ST7各1株（4．17％）。结论鄂尔多斯地区蒙古族人群MRSA菌株以SCCmec Ⅱ型和SCCmecⅢ型为主。
목적：탐토악이다사지구몽고족인군내갑양서림금황색포도구균（MRSA）적분자류행병학특점，명학본지구MRSA기인형별급기분포규률。방법수집2009년1월지2011년8월림상분리적MRSA균주54주。응용다중취합매련반응（PCR）대MRSA균주진행포도구균염색체mec（SCCmec）기인분형、살백세포독소（PVL）독력기인（pvl）화다위점서렬분형（MLST）검측。결과 MRSA균주적SCCmec Ⅰ～Ⅴ기인분형분별점0．00％、50．00％、46．30％、1．85％화1．85％；검측출1주pvl기인양성；24주MRSA균주MLST분형현시，ST23913주（54．17％），ST59주（37．50％），ST59화ST7각1주（4．17％）。결론악이다사지구몽고족인군MRSA균주이SCCmec Ⅱ형화SCCmecⅢ형위주。
Objective To investigate the molecular prevalence characteristics of methicillin-resistant Staphylococcus aureus (MRSA)from Mongolian in Ordos,and to investigate the genotypes and distribution pattern of MRSA isolates. Methods A total of 54 MRSA isolates were collected from January 2009 to August 201 1 .The multiplex polymerase chain reaction(PCR)was used to determine Staphylococcal cassette chromosome mec (SCCmec)genotypes,Panton-Valentine leukocidin (PVL)gene (pvl)and multi-locus sequence typing (MLST)of MRSA isolates.Results The main SCCmec Ⅰ-Ⅴ genotypes of MRSA isolates,accounted for 0.00%,50.00%,46.30%,1 .85% and 1 .85%, respectively.The pvl gene had been discovered in only 1 isolate.Among 24 MRSA isolates,MLST results showed that 13 isolates were ST239(54.17%),9 isolates were ST5(37.50%),1 isolate was ST59 (4.17%),and 1 isolate was ST7 (4.1 7%).Conclusions The main genotypes of MRSA are SCCmecⅡand SCCmecⅢfrom Mongolian in Ordos.