检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2014年
2期
97-99,103
,共4页
刘辰庚%夏鸣%王金玲%李蕾%王培昌
劉辰庚%夏鳴%王金玲%李蕾%王培昌
류신경%하명%왕금령%리뢰%왕배창
自身抗体%碳酸酐酶Ⅱ%碳酸酐酶Ⅲ%充血性心力衰竭
自身抗體%碳痠酐酶Ⅱ%碳痠酐酶Ⅲ%充血性心力衰竭
자신항체%탄산항매Ⅱ%탄산항매Ⅲ%충혈성심력쇠갈
Auto-antibody%Carbonic anhydrase Ⅱ%Carbonic anhydrase Ⅲ%Congestive heart failure
目的:使用已建立的方法检测充血性心力衰竭(CHF)患者血清中的抗碳酸酐酶Ⅱ和Ⅲ自身抗体、抗氧化物及细胞因子水平,初步评价其临床意义。方法选取58例3个月~1年前曾发生心肌梗死的CHF患者和58名健康对照者,测定其血清抗碳酸酐酶Ⅱ和Ⅲ自身抗体、红细胞沉降率(ESR)、超氧化物歧化酶(SOD)等5种抗氧化物和白细胞介素2(IL-2)等5种细胞因子的水平。结果 CHF患者血清抗碳酸酐酶Ⅱ自身抗体和ESR水平显著高于对照组(P<0.05),SOD、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)和总抗氧化能力(TAC)显著低于对照组(P<0.05),丙二醛(MDA)水平显著高于对照组(P<0.05)。CHF患者血清的IL-2和白细胞介素17(IL-17)水平较对照组显著升高(P<0.05),白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和γ干扰素(IFN-γ)与对照组差异无统计学意义(P>0.05)。结论 CHF患者可能存在自身免疫的现象,IL-2、IL-17和抗氧化能力的检测可能作为判断抗碳酸酐酶Ⅱ抗体出现的指标。
目的:使用已建立的方法檢測充血性心力衰竭(CHF)患者血清中的抗碳痠酐酶Ⅱ和Ⅲ自身抗體、抗氧化物及細胞因子水平,初步評價其臨床意義。方法選取58例3箇月~1年前曾髮生心肌梗死的CHF患者和58名健康對照者,測定其血清抗碳痠酐酶Ⅱ和Ⅲ自身抗體、紅細胞沉降率(ESR)、超氧化物歧化酶(SOD)等5種抗氧化物和白細胞介素2(IL-2)等5種細胞因子的水平。結果 CHF患者血清抗碳痠酐酶Ⅱ自身抗體和ESR水平顯著高于對照組(P<0.05),SOD、穀胱甘肽過氧化物酶(GPx)、過氧化氫酶(CAT)和總抗氧化能力(TAC)顯著低于對照組(P<0.05),丙二醛(MDA)水平顯著高于對照組(P<0.05)。CHF患者血清的IL-2和白細胞介素17(IL-17)水平較對照組顯著升高(P<0.05),白細胞介素6(IL-6)、腫瘤壞死因子α(TNF-α)和γ榦擾素(IFN-γ)與對照組差異無統計學意義(P>0.05)。結論 CHF患者可能存在自身免疫的現象,IL-2、IL-17和抗氧化能力的檢測可能作為判斷抗碳痠酐酶Ⅱ抗體齣現的指標。
목적:사용이건립적방법검측충혈성심력쇠갈(CHF)환자혈청중적항탄산항매Ⅱ화Ⅲ자신항체、항양화물급세포인자수평,초보평개기림상의의。방법선취58례3개월~1년전증발생심기경사적CHF환자화58명건강대조자,측정기혈청항탄산항매Ⅱ화Ⅲ자신항체、홍세포침강솔(ESR)、초양화물기화매(SOD)등5충항양화물화백세포개소2(IL-2)등5충세포인자적수평。결과 CHF환자혈청항탄산항매Ⅱ자신항체화ESR수평현저고우대조조(P<0.05),SOD、곡광감태과양화물매(GPx)、과양화경매(CAT)화총항양화능력(TAC)현저저우대조조(P<0.05),병이철(MDA)수평현저고우대조조(P<0.05)。CHF환자혈청적IL-2화백세포개소17(IL-17)수평교대조조현저승고(P<0.05),백세포개소6(IL-6)、종류배사인자α(TNF-α)화γ간우소(IFN-γ)여대조조차이무통계학의의(P>0.05)。결론 CHF환자가능존재자신면역적현상,IL-2、IL-17화항양화능력적검측가능작위판단항탄산항매Ⅱ항체출현적지표。
Objective To determine serum anti-carbonic anhydrase Ⅱ and Ⅲ auto-antibodies,the level of antioxidants and cytokines in patients with congestive heart failure (CHF)by established methods,and to evaluate primarily the clinical significance. Methods The serum anti-carbonic anhydrase Ⅱ and Ⅲ auto-antibodies, erythrocyte sedimentation rate(ESR),the levels of 5 kinds of cytokines [like interleukin 2 (IL-2 )]and 5 kinds of antioxidants [like superoxide dismutase (SOD)]from 58 patients with CHF in recent 3 months to 1 year were determined.Results The level of serum anti-carbonic anhydrase Ⅱ auto-antibody in CHF patients was significantly higher than that in control group (P<0.05 ).The levels of SOD,glutathione peroxidase (GPx),catalase (CAT)and total antioxidant capacity (TAC)in CHF patients were significantly lower than those in control group (P<0.05),while methanedicarboxylic aldehyde (MDA)level was significantly higher than that in control group(P<0.05).There was no statistical significance on the levels of interleukin (IL-6 ),tumor necrosis factor-alpha (TNF-α)and interferon-gamma (IFN-γ)between CHF patients and control group (P>0.05 ),while IL-2 and interleukin 1 7 (IL-1 7 )levels were significantly higher than those in control group (P<0.05 ).Conclusions There may be autoimmune phenomenon in CHF patients.IL-2,IL-1 7 and antioxidant capacity may be used as the markers for the diagnosis of anti-carbonic anhydrase Ⅱ auto-antibody.