CAJ | 학술논문

试验目的是从肉鸡的肠道内容物及排泄物中筛选出具有铅抗性的细菌，用于重金属铅的去除。采用涂布平板技术从采集的样品中共分离筛选到8株具有较好铅抗性和吸附性的菌株，其中JT1菌株对铅的抗性（800 mg/L）和去除能力（66．95％）最高。通过培养特征、形态观察、生理生化鉴定、16S rDNA序列分析等方法证明，所得JT1菌株为屎肠球菌（Enterococcus faecium）。该菌株可以在10～45℃、pH 4．5～9．6、6．5％NaCl溶液、0．8％胆盐、0．1％胰蛋白酶浓度条件下生长良好。
시험목적시종육계적장도내용물급배설물중사선출구유연항성적세균，용우중금속연적거제。채용도포평판기술종채집적양품중공분리사선도8주구유교호연항성화흡부성적균주，기중JT1균주대연적항성（800 mg/L）화거제능력（66．95％）최고。통과배양특정、형태관찰、생리생화감정、16S rDNA서렬분석등방법증명，소득JT1균주위시장구균（Enterococcus faecium）。해균주가이재10～45℃、pH 4．5～9．6、6．5％NaCl용액、0．8％담염、0．1％이단백매농도조건하생장량호。
The purpose of this study was to isolate and identify lead -resistant lactic acid bacteria from chicken intestinal content and excreta for removal of lead .Eight strains were isolated from the collected sam-ples by spread plate technique using MRS agar media supplemented with lead .The selected JT 1 strain showed the highest lead resistant (800 mg/L) and removal efficiency (66.95%).The results of morphology observa-tion, differential cultivation, physiological and biochemical identification and 16S ribosomal deoxyribonucleic acid sequencing data showed it could be identified as Enterococcus faecium, and it could grow well under the conditions of 10~45℃, pH value as 4.5~9.6, 6.5%NaCl, 0.8%bile salts and 0.1%trypsase.