山东农业科学
山東農業科學
산동농업과학
SHANGDONG AGRICULTURAL SCIENCES
2014年
2期
11-12,16
,共3页
陈新%刘洪对%贾建云%徐丽%张力思%刘庆忠
陳新%劉洪對%賈建雲%徐麗%張力思%劉慶忠
진신%류홍대%가건운%서려%장력사%류경충
核桃%总RNA%Trizol%EASYspin%Qiagen
覈桃%總RNA%Trizol%EASYspin%Qiagen
핵도%총RNA%Trizol%EASYspin%Qiagen
Juglans regia L.%Total RNA%Trizol%EASYspin RN09%Qiagen
通过对比Trizol、EASYspin和Qiagen 3种试剂盒提取方法，探讨适合核桃叶片总RNA提取的有效方法。结果表明EASYspin试剂盒提取的RNA纯度较高、完整性好，试验用时较短，且试剂盒成本较低。
通過對比Trizol、EASYspin和Qiagen 3種試劑盒提取方法，探討適閤覈桃葉片總RNA提取的有效方法。結果錶明EASYspin試劑盒提取的RNA純度較高、完整性好，試驗用時較短，且試劑盒成本較低。
통과대비Trizol、EASYspin화Qiagen 3충시제합제취방법，탐토괄합핵도협편총RNA제취적유효방법。결과표명EASYspin시제합제취적RNA순도교고、완정성호，시험용시교단，차시제합성본교저。
Three methods of Trizol , EASYspin RN09 and Qiagen were compared in this paper to find out the effective method to extract total RNA from walnut leaves .The results showed that the RNA extracted with EASYspin RN09 kit had the higher purity and good integrality , and this method saved time and cost .
