CAJ | 학술논문

建立高效液相色谱-二极管阵列检测器（ HPLC-DAD）定性定量分析油橄榄叶中橄榄苦苷方法。采用反相高效液相色谱法检测油橄榄叶中的橄榄苦苷，色谱柱为Akasil C18，流动相为23％乙腈-水溶液，检测波长为230 nm，柱温为35℃，流速为1 mL/min。结果表明：油橄榄苦苷在0．0125～0．4 mg/mL内与其峰面积呈良好的线性关系，相关系数R2＝0．9999，根据橄榄苦苷的线性回归方程得出油橄榄叶中橄榄苦苷含量为0．39％。
건립고효액상색보-이겁관진렬검측기（ HPLC-DAD）정성정량분석유감람협중감람고감방법。채용반상고효액상색보법검측유감람협중적감람고감，색보주위Akasil C18，류동상위23％을정-수용액，검측파장위230 nm，주온위35℃，류속위1 mL/min。결과표명：유감람고감재0．0125～0．4 mg/mL내여기봉면적정량호적선성관계，상관계수R2＝0．9999，근거감람고감적선성회귀방정득출유감람협중감람고감함량위0．39％。
The purpose of the study is to establish a method for qualitative and quantitative analysis oleuropein in olive leaves by high-performance liquid chromatography -diode array detector ( HPLC-DAD) .Reversed-phase HPLC is used to determine the contents of oleuropein in olive leaves by DAD with Akasil C 18 column, using 23% acetonitrile as mobile phase under UV light of 230 nm at 35℃and 1.0 mL/min.The Results indicate that the calibration curves of oleuropein have good linearity over the range from 0.0125 to 0.4 mg/mL with a correlation coeffient of 0.999 9 , the yield of oleuropein in olive leaves is 0.39%.