激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2014年
2期
70-72
,共3页
李凤%吕凯%龚标%代恩泽%李学智%黄思琴%汪莹
李鳳%呂凱%龔標%代恩澤%李學智%黃思琴%汪瑩
리봉%려개%공표%대은택%리학지%황사금%왕형
局灶性脑梗死%电针%RhoA,ROCK
跼竈性腦梗死%電針%RhoA,ROCK
국조성뇌경사%전침%RhoA,ROCK
Cerebral ischemia%Electroacupuncture%RhoA and ROCK
目的：观察大鼠局灶性脑梗死后大脑皮质RhoA和ROCK(Rho-associated kinases)的表达以及电针刺激对其表达的影响。方法：将成年健康雄性SD大鼠40只，随机分为正常组，假手术组，模型组和电针组，每组十只。模型组和电针组利用改进后的Longa方法制作大脑中动脉闭塞（middle cerebral artery occlusion,MCAO)模型，电针组在大鼠麻醉苏醒后90min进行电针刺激，每天一次，连续针刺14天。每组分别在1d,3d,7d,14d四个时间点进行神经行为学评分。14天时运用免疫组织化学法观测缺血区大脑皮质中RhoA和ROCK的分布和表达，利用免疫印迹法检测缺血区侧大脑皮质RhoA和ROCK的蛋白表达。结果：模型组大鼠神经行为学评分明显高于正常组和假手术组(p<0.01),14天后电针组的神经行为学评分明显低于同时段模型组(p<0.05)；免疫组化结果显示，电针组的RhoA和ROCK表达较模型组明显减少（p﹤0.05）；Western blot检测结果显示，电针组RhoA蛋白和ROCK蛋白的表达量较模型组RhoA蛋白和ROCK蛋白明显减少（p＜0.05）结论：大鼠局灶性脑梗死后脑损区皮质RhoA与ROCK表明显上调，给予电针干预后可降低其表达，这可能是电针促进MCAO大鼠脑损区轴突再生的机制之一。
目的：觀察大鼠跼竈性腦梗死後大腦皮質RhoA和ROCK(Rho-associated kinases)的錶達以及電針刺激對其錶達的影響。方法：將成年健康雄性SD大鼠40隻，隨機分為正常組，假手術組，模型組和電針組，每組十隻。模型組和電針組利用改進後的Longa方法製作大腦中動脈閉塞（middle cerebral artery occlusion,MCAO)模型，電針組在大鼠痳醉囌醒後90min進行電針刺激，每天一次，連續針刺14天。每組分彆在1d,3d,7d,14d四箇時間點進行神經行為學評分。14天時運用免疫組織化學法觀測缺血區大腦皮質中RhoA和ROCK的分佈和錶達，利用免疫印跡法檢測缺血區側大腦皮質RhoA和ROCK的蛋白錶達。結果：模型組大鼠神經行為學評分明顯高于正常組和假手術組(p<0.01),14天後電針組的神經行為學評分明顯低于同時段模型組(p<0.05)；免疫組化結果顯示，電針組的RhoA和ROCK錶達較模型組明顯減少（p﹤0.05）；Western blot檢測結果顯示，電針組RhoA蛋白和ROCK蛋白的錶達量較模型組RhoA蛋白和ROCK蛋白明顯減少（p＜0.05）結論：大鼠跼竈性腦梗死後腦損區皮質RhoA與ROCK錶明顯上調，給予電針榦預後可降低其錶達，這可能是電針促進MCAO大鼠腦損區軸突再生的機製之一。
목적：관찰대서국조성뇌경사후대뇌피질RhoA화ROCK(Rho-associated kinases)적표체이급전침자격대기표체적영향。방법：장성년건강웅성SD대서40지，수궤분위정상조，가수술조，모형조화전침조，매조십지。모형조화전침조이용개진후적Longa방법제작대뇌중동맥폐새（middle cerebral artery occlusion,MCAO)모형，전침조재대서마취소성후90min진행전침자격，매천일차，련속침자14천。매조분별재1d,3d,7d,14d사개시간점진행신경행위학평분。14천시운용면역조직화학법관측결혈구대뇌피질중RhoA화ROCK적분포화표체，이용면역인적법검측결혈구측대뇌피질RhoA화ROCK적단백표체。결과：모형조대서신경행위학평분명현고우정상조화가수술조(p<0.01),14천후전침조적신경행위학평분명현저우동시단모형조(p<0.05)；면역조화결과현시，전침조적RhoA화ROCK표체교모형조명현감소（p﹤0.05）；Western blot검측결과현시，전침조RhoA단백화ROCK단백적표체량교모형조RhoA단백화ROCK단백명현감소（p＜0.05）결론：대서국조성뇌경사후뇌손구피질RhoA여ROCK표명현상조，급여전침간예후가강저기표체，저가능시전침촉진MCAO대서뇌손구축돌재생적궤제지일。
Objective:To observe the expression of RhoA and ROCK on MCAO ratS’ cortex and the effects of electroacupunc-ture (EA) intervention on its expression. Methods 40 healthy adult male Sprague Dawley (SD) rats were equally randomized into Control group,Sham-operation group , Model group and EA group. The model group and EA group with middle cerebral artery occlu-sion were successfully established by an improved Longa procedure.EA was used 90min after anesthesia revival for the EA group.The rats in the EA group accepted the EA treatment for 14 days,once a day. After 14days, immunohistochemistry and Western blot were used to detect expression of the RhoA and ROCK in the brain.Results Compared to the model group,the.neurological.dysfunction.of. EAlgroupkrecoveredjsignificantly(p<0.05).Immunohistochemical staining and western blot both indicated that the expression levels of RhoA and ROCK in EA group were less than those in model group (p﹤0.05 ).Conclusion After focal cerebral infarction RhoA and ROCK expresstionmonmratsncortexnwould;significantly.increased.Giving electroacupuncture interventions can down regulate its ex-pression, this may be one of the mechanisms of EA promoting axonal regeneration after cerebral infarction.
