CAJ | 학술논문

[目的]从种植野生稻的稻田土壤中分离筛选对环境无污染、具有高效生物絮凝活性的菌株．[方法]采用VM培养基从药用野生稻中分离135个菌株，以发酵液对高岭土悬液絮凝效果为指标，筛选到絮凝活性高的菌株YH39．[结果和结论]16S rDNA序列分析表明，该菌株与越南伯克霍尔德菌Burkholderia vietnamiensis有98.6％的相似性．YH39产絮凝剂最佳碳源为葡萄糖，最佳氮源为硝酸钾．发酵动力学研究表明，菌株YH39发酵培养16 h，菌体浓度和絮凝活性达到最高值．对絮凝剂成分分析表明，絮凝剂为多糖，具有很好的耐热性，在60℃下加热30 min，絮凝活性不改变．应用研究结果表明，此絮凝剂对3种印染废水均有很好的絮凝效果，不仅可以高效地降低废水的CODCr ，还有很好的脱色效果．
[목적]종충식야생도적도전토양중분리사선대배경무오염、구유고효생물서응활성적균주．[방법]채용VM배양기종약용야생도중분리135개균주，이발효액대고령토현액서응효과위지표，사선도서응활성고적균주YH39．[결과화결론]16S rDNA서렬분석표명，해균주여월남백극곽이덕균Burkholderia vietnamiensis유98.6％적상사성．YH39산서응제최가탄원위포도당，최가담원위초산갑．발효동역학연구표명，균주YH39발효배양16 h，균체농도화서응활성체도최고치．대서응제성분분석표명，서응제위다당，구유흔호적내열성，재60℃하가열30 min，서응활성불개변．응용연구결과표명，차서응제대3충인염폐수균유흔호적서응효과，불부가이고효지강저폐수적CODCr ，환유흔호적탈색효과．
[Objective] This study aimed at isolating and screening of efficiently biological flocculation strains from the paddy field of the planted wild rice .[Method]A high bioflocculant strain YH 39 was ob-tained from the screening of kaolin suspension flocculation effects of 135 strains isolated from Oryza offici-nalis on VM medium.[Result and conclusion]16S rDNA sequences analysis showed that the strain YH39 was closely related to the known species Burkholderia cepacia with 98.6%similarity.The best car-bon and nitrogen sources for producing flocculation were glucose and potassium nitrate , respectively .The fermentation kinetics analysis showed that strain YH 39 reached the highest values of biomass concentra-tion and flocculating activity in 16 h.Flocculant composition analysis indicated that the bioflocculant components were polysaccharide with good heat resistance , which was heated at 60℃for 30 min without losing flocculating activity .The application of bioflocculation of three kinds of dyeing wastewater show that the fermentation liquid has good flocculating effects on dyeing wastewater with effective decolorization and reduces COD Cr in dyeing wastewater .